Activation of LH receptors expressed in GnRH neurons stimulates cyclic AMP production and inhibits pulsatile neuropeptide release

Endocrinology
N MoresK J Catt

Abstract

The GT1-7 cell line of immortalized GnRH neurons has been shown to express receptors for GnRH, LH, and prolactin, as well as a variety of other hormones and transmitters. Treatment of GT1-7 cells with hCG caused a dose-dependent increase in cAMP production, with a rapid increase during the first 15 min and a subsequent decrease that was prevented by pre-treatment with pertussis toxin. Furthermore, the stimulatory effect of cholera toxin on cAMP production was inhibited by hCG in a dose-dependent manner. These data indicate that the LH receptors expressed in GT1-7 cells are coupled to adenylyl cyclase both stimulatory (Gs) and inhibitory (Gi) proteins. In perifused cell cultures, treatment with forskolin and 8-bromo cAMP increased the amplitude of spontaneous GnRH release. However, treatment with nanomolar concentrations of hCG abolished pulsatile GnRH release from both GT1-7 cells and rat hypothalamic cells. The similarity of hCG action on pulsatile GnRH release to that of extracellular Ca2+ depletion and calcium channel antagonists, and its partial resistance to potassium-induced depolarization, suggest that it results from inhibition of plasma-membrane ion channel activity. It is probable that the inhibitory action of hCG on ...Continue Reading

Citations

Feb 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Eun-Jin YangDarcy B Kelley
Oct 6, 2005·Reproductive Biology and Endocrinology : RB&E·Mohamed F M MitwallyMichael P Diamond
Jan 23, 2003·Reproductive Biomedicine Online·Efstratios M Kolibianakis, Paul Devroey
Sep 11, 2009·Trends in Endocrinology and Metabolism : TEM·Lazar Z KrsmanovicKevin J Catt
Aug 31, 2001·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·R I Weiner, A Charles
Oct 12, 2010·Molecular and Cellular Endocrinology·Betina GonzalezSusana B Rulli
Aug 24, 2018·Systems Biology in Reproductive Medicine·Maurice Andre RecanatiRobert A Welch

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