Activation of liver X receptors inhibits cadmium-induced apoptosis of human renal proximal tubular cells

Toxicology Letters
Somsak FongsupaSunhapas Soodvilai

Abstract

Liver X receptors (LXRs) including LXRα and LXRβ are members of the nuclear receptor superfamily of ligand-activated transcription factors, which are expressed in high metabolic organs such as the liver, kidney, and adipose tissue. LXRs have been shown to act as antioxidants and anti-inflammatory in several organs. The present study investigated the effects of LXR activation on cadmium-induced cell death in renal proximal tubular cells. Treating human renal proximal tubular cells, HK-2 cells, with 20μM CdCl2 for 24h led to cell death via apoptosis but not necrosis. Interestingly, pretreating HK-2 cells with T0901317, a LXR agonist, significantly inhibited the apoptotic cell death induced by CdCl2. The protective effect of T0901317 was eliminated by incubation with fenofibrate, a LXR antagonist, indicating that the effect of T0901317 on cadmium-induced apoptotic cell death was mediated by LXR activation. In addition, the effect of CdCl2 was attenuated by a reactive oxygen species (ROS) scavenger, N-acetyl-l-cysteine (NAC). An increase in ROS induced by CdCl2 was mediated by inhibition of catalase but not superoxide dismutase (SOD) which was attenuated by T0901317. Western blot analysis revealed that CdCl2 stimulated expression o...Continue Reading

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Citations

Oct 13, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Yongxia ChengGuibo Liu
Mar 1, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ting XiongJian Tu
Nov 12, 2017·Experimental Physiology·Pattira ChonlaketSunhapas Soodvilai
Aug 2, 2018·Journal of Mammary Gland Biology and Neoplasia·Xiaoyu HuYunhe Fu
Feb 9, 2019·Environmental Science and Pollution Research International·Sumita HalderBasu D Banerjee

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