PMID: 376774May 1, 1979Paper

Activation of macrophages in vivo and in vitro. Correlation between hydrogen peroxide release and killing of Trypanosoma cruzi

The Journal of Experimental Medicine
C F NathanZ Cohn

Abstract

As reported previously, mouse peritoneal macrophages could be activated to kill intracellular trypomastigotes of Trypanosoma cruzi, the agent of Chagas' disease, in either of two ways: by immunizing and boosting the mice (3), or by culturing resident or inflammatory macrophages in spleen cell factor(s) (SCF) in vitro (2). Macrophages activated in vivo became less trypanocidal with time in culture, and cells activated in vitro lost trypanocidal capacity when CSF was removed (2). In the present study, the ability of macrophages to release H2O2 in response to phorbol myristate acetate (PMA) could be induced in vivo and in vitro, and reversed in vitro, in a manner correlating closely with changes in trypanocidal activity. Macrophages could be activated in vitro with SCF in a time-dependent and dose-dependent fashion, so that they released as much H2O2 as macrophages activated in vivo. The sensitivity of epimastigotes and trypomastigotes to enzymatically generated H2O2 suggested that the generation of H2O2 by activated macrophages could be plausible explanation for their trypanocidal activity. Of the biochemical correlates of macrophage activation reported to date, increased ability to release H2O2 seems most closely allied to enhan...Continue Reading

References

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Citations

Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·C F Nathan
Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·D B Lowrie
Jan 1, 1985·Developmental and Comparative Immunology·M NakamuraT Nomura
May 1, 1993·Developmental and Comparative Immunology·K L Bell, V J Smith
Jan 1, 1985·Comparative Immunology, Microbiology and Infectious Diseases·E Schneider, M Dy
Jun 1, 1981·Immunopharmacology·R G DevlinH Dougherty
Oct 1, 1981·Molecular and Biochemical Parasitology·H W MurrayW A Scott
Aug 1, 1991·Molecular and Biochemical Parasitology·W P Michalski, S J Prowse
Jan 1, 1983·International Journal of Immunopharmacology·H P HartungU Hadding
Jan 1, 1984·International Journal of Immunopharmacology·Y KumazawaK Nomoto
Jan 1, 1988·Free Radical Biology & Medicine·P Bellavite
Feb 1, 1991·Research in Immunology·N Russo, N Starobinas
Oct 17, 2001·Developmental and Comparative Immunology·N F NeumannM Belosevic
Jul 31, 2003·Pharmacology & Therapeutics·Ian A Clark, William B Cowden
Jul 15, 1998·Molecular and Biochemical Parasitology·R ChandrashekarG J Weil
May 1, 1986·Annales De L'Institut Pasteur. Immunology·C F Nathan
Jan 1, 1997·Free Radical Biology & Medicine·T A SchererW M Abraham
Oct 23, 1980·The New England Journal of Medicine·E R Unanue
Dec 23, 1982·The New England Journal of Medicine·W C Van VoorhisZ A Cohn
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K P Chang, J W Chiao
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T M Aune, C W Pierce
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·Y TanakaB R Bloom
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T P KoestlerG Poste
Oct 1, 1979·The Journal of Experimental Medicine·H W MurrayZ A Cohn
Jun 1, 1980·The Journal of Experimental Medicine·G R OldsA A Mahmoud

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