Activation of mammalian cytoplasmic dynein in multimotor motility assays

Journal of Cell Science
Gina A MonzonStefan Diez

Abstract

Long-range intracellular transport is facilitated by motor proteins, such as kinesin-1 and cytoplasmic dynein, moving along microtubules (MTs). These motors often work in teams for the transport of various intracellular cargos. Although transport by multiple kinesin-1 motors has been studied extensively in the past, collective effects of cytoplasmic dynein are less well understood. On the level of single molecules, mammalian cytoplasmic dynein is not active in the absence of dynactin and adaptor proteins. However, when assembled into a team bound to the same cargo, processive motility has been observed. The underlying mechanism of this activation is not known. Here, we found that in MT gliding motility assays the gliding velocity increased with dynein surface density and MT length. Developing a mathematical model based on single-molecule parameters, we were able to simulate the observed behavior. Integral to our model is the usage of an activation term, which describes a mechanical activation of individual dynein motors when being stretched by other motors. We hypothesize that this activation is similar to the activation of single dynein motors by dynactin and adaptor proteins.This article has an associated First Person intervi...Continue Reading

References

Oct 12, 2000·Nature Cell Biology·M J SchnitzerS M Block
Nov 30, 2007·Cell·Arne GennerichRonald D Vale
Jun 10, 2009·Physical Chemistry Chemical Physics : PCCP·Adam G LarsonSarah E Rice
Apr 20, 2010·Current Biology : CB·Peter BielingThomas Surrey
May 5, 2010·The Journal of Cell Biology·Christian Hentrich, Thomas Surrey
Jun 7, 2011·Biophysical Journal·Felix RuhnowStefan Diez
May 24, 2014·Biophysical Journal·Youské ShimizuKazuhiro Oiwa
Jul 6, 2014·The EMBO Journal·Max A SchlagerAndrew P Carter
Jul 17, 2014·Biophysical Journal·Lara ScharrelStefan Diez
Jul 19, 2014·Science·Richard J McKenneyRonald D Vale
Aug 12, 2014·Nature Communications·Frank B ClearyAhmet Yildiz
Oct 1, 2014·Nature Cell Biology·Takayuki TorisawaKen'ya Furuta
Feb 12, 2015·Nature Communications·Matthew P NicholasArne Gennerich
Apr 11, 2017·Nature Structural & Molecular Biology·Katerina ToropovaAnthony J Roberts

❮ Previous
Next ❯

Citations

Jun 17, 2020·Journal of Cell Science·Marcus BraunZdenek Lansky
Sep 25, 2020·Royal Society Open Science·Naruemon RueangkhamRhoda J Hawkins
Oct 28, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yan ZhouBin Qu
Aug 28, 2021·International Journal of Molecular Sciences·Gal HalbiAnne Bernheim-Groswasser

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Ken'ya FurutaHiroaki Kojima
Protein Science : a Publication of the Protein Society
Anjneya TakshakAmbarish Kunwar
© 2022 Meta ULC. All rights reserved