Activation of mitogen-activated protein kinase in freshly isolated rat hepatocytes by both a calcium- and a protein kinase C-dependent pathway

Metabolism: Clinical and Experimental
A Romanelli, G van de Werve

Abstract

In the present study, we investigated the role of calcium and protein kinase C (PKC) in the activation of mitogen-activated protein kinase (MAPK) in isolated rat hepatocytes. We found that the glycogenolytic hormone norepinephrine (NE), acting through the alpha1-adrenergic receptor and the G protein Gq, was able to induce a dose- and time-dependent activation of MAPK in hepatocytes. Vasopressin, which acts through a different receptor but also through stimulation of the Gq-dependent pathway, also caused a twofold activation of MAPK. Activation of MAPK by both agonists required the presence of free extracellular calcium and was blocked by the specific PKC inhibitor, Ro 31-8220. MAPK activation was also induced by phorbol myristate acetate (PMA), confirming that a PKC-dependent pathway exists for MAPK activation in liver. Furthermore, calcium-mobilizing agents such as thapsigargin and ionomycin were able to induce an activation of MAPK by a PKC-independent pathway that was totally abolished by preincubation of cells with EGTA. A second pathway for MAPK activation that relies solely on calcium may therefore exist. Ro 31-8220 did not affect phosphorylase activation by NE, vasopressin, thapsigargin, and ionomycin, indicating that PK...Continue Reading

References

Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
Jan 1, 1980·Toxicology·G van de Werve
Oct 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P GinèsR A Nemenoff
Jul 21, 1995·The Journal of Biological Chemistry·B E HawesR J Lefkowitz
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·W J KochR J Lefkowitz
Apr 1, 1994·Journal of Cellular Biochemistry·G L JohnsonS Winitz
Nov 1, 1993·Biochemical Society Transactions·M PeakL Agius
Oct 1, 1993·The American Journal of Physiology·A KribbenR A Nemenoff
Jan 28, 1993·Nature·M J Berridge
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·L B Rosen, M E Greenberg
May 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T AdachiY Nozawa

❮ Previous
Next ❯

Citations

Jul 11, 2002·European Journal of Pharmacology·Yoshihumi KawanabeTomoh Masaki
Feb 22, 2002·Biochemical and Biophysical Research Communications·Laila S NilssenDagny Sandnes
Sep 12, 2015·Molecular and Cellular Biochemistry·Hyun Young JeongHyun Ju Kwon
Sep 27, 2005·Cellular Signalling·González-Yanes Carmen, Sánchez-Margalet Víctor
Mar 15, 2006·British Journal of Pharmacology·Kristiana Kandere-GrzybowskaTheoharis C Theoharides
Sep 18, 2002·The Journal of Pharmacology and Experimental Therapeutics·Xiuxiang JiaoWilliam B Jeffries
Apr 20, 2000·The Journal of Biological Chemistry·J M LindquistJ Nedergaard
Aug 12, 1999·European Journal of Pharmacology·H Zhong, K P Minneman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.