PMID: 9182692Jun 1, 1997Paper

Activation of mitogen-activated protein kinase and p70S6 kinase is not correlated with cerebellar granule cell survival

The Biochemical Journal
F J Gunn-MooreJ M Tavaré

Abstract

We have investigated the role of mitogen-activated protein (MAP) kinase in the survival of cerebellar granule cells in primary culture. Brain-derived neurotrophic factor (BDNF) and insulin, but not epidermal growth factor (EGF), promoted the survival of P6 cerebellar granule neurons. BDNF promoted a sustained activation of MAP kinase, whereas that induced by EGF was only transient. Insulin promoted a small but transient activation of MAP kinase that was completely blocked by PD98059, an inhibitor of MAP kinase kinase activation. PD98059 had no effect on the insulin- or BDNF-induced survival of cerebellar granule cells. We also investigated the role of p70S6 kinase in survival. The activation of p70S6 kinase by EGF was transient, whereas BDNF and insulin promoted a sustained activation of p70S6 kinase. Rapamycin, which blocked p70S6 kinase activation, had no effect on the BDNF- or insulin-induced survival of cerebellar granule cells. We conclude that sustained activation of MAP kinase is not correlated with the survival response of cerebellar granule cells; indeed insulin-mediated survival is independent of MAP kinase. Survival of cerebellar granule cells is also independent of the activation of p70S6 kinase.

Citations

Apr 10, 2003·Cell and Tissue Research·J H WeishauptM Bähr
Dec 4, 2002·Journal of Cellular Biochemistry·Edward Chaum
Dec 17, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mei LiLi-Hua Zhou
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