Activation of mitogen-activated protein kinases and AP-1 by polysaccharide isolated from the radix of Platycodon grandiflorum in RAW 264.7 cells

International Immunopharmacology
Yeo Dae YoonHwan Mook Kim

Abstract

The root of Platycodon grandiflorum has been widely used for the treatment of various diseases in oriental medicine. Our previous study showed that the PG, a polysaccharide isolated from P. grandiflorum, activates macrophages via Toll-like receptor 4 (TLR4). However, the associated biological mechanisms are not fully understood. To elucidate the molecular mechanism responsible for the macrophage activation, we investigated the effect of PG on the activity of mitogen-activated protein kinases (MAPKs) and activator protein-1 (AP-1) in RAW 264.7 cells, a murine macrophage cell line. Treatment of RAW 264.7 cells with PG produced a marked induction of AP-1 DNA binding activity. Moreover, all three MAPKs were activated by PG, and PG-induced activation of MAPKs was abrogated by the treatment of PD98059, curcumin, and SB203580, specific inhibitors of MEK-1/2, stress-activated protein kinases/jun N-terminal kinase (SAPK/JNK), and p38 MAP kianse, respectively. The induction of AP-1 DNA binding activity by PG was also inhibited by these MAPK inhibitors. Moreover, supershift analysis identified that JunB and Fra-1 are major components involved in the PG-mediated induction of AP-1 DNA binding. Additionally, curcumin and SB203580 suppressed ...Continue Reading

References

May 1, 1992·Chemical & Pharmaceutical Bulletin·M OkaH Komura
May 1, 1996·International Journal of Immunopharmacology·H M KimI D Yoo
Jun 1, 1996·International Journal of Immunopharmacology·C KerékgyártóJ Fachet
Jan 1, 1997·Annual Review of Immunology·J MacMickingC Nathan
Aug 7, 1999·Immunopharmacology·Y J JeonH M Kim
Feb 19, 2000·Current Opinion in Immunology·K V Anderson
Mar 21, 2001·Cellular Signalling·M Guha, N Mackman
May 22, 2001·International Immunopharmacology·Y J JeonH M Kim
Sep 14, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K Takeda, S Akira
May 4, 2002·Nature Cell Biology·Eitan Shaulian, Michael Karin
Jun 12, 2002·Free Radical Biology & Medicine·Karen E IlesHenry Jay Forman
Oct 14, 2003·Trends in Immunology·Paul J HertzogJohn A Hamilton

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Citations

Jul 16, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mi Jeong ParkSang-Bae Han
Nov 18, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Line Edwige MengomePatrice Lerouge
Jan 5, 2011·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Min-Soo KimHyunsu Bae
Dec 23, 2009·Biochemical and Biophysical Research Communications·Zhi-Qiang ChangSeung-Chun Park
Dec 21, 2011·Chemical Biology & Drug Design·M Ahmed MesaikM Iqbal Choudhary
Nov 22, 2005·Biochemical and Biophysical Research Communications·Shu-Ling FuYu-Jing Huang
Jun 13, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hyung Sook KimSang-Bae Han
Feb 6, 2013·International Immunopharmacology·Igor A SchepetkinMark T Quinn
Jan 8, 2013·Journal of Ethnopharmacology·Koffi KouakouMark T Quinn
Dec 13, 2006·International Immunopharmacology·Jee Youn KimSang-Bae Han
Apr 9, 2009·Journal of Pharmacological Sciences·Jee Youn KimSang-Bae Han
Jun 5, 2016·Carbohydrate Polymers·Guodong WangKaoshan Chen
Jun 26, 2007·Bioscience, Biotechnology, and Biochemistry·Ah-Jin KimJae-Kwan Hwang
Nov 10, 2010·Expert Opinion on Therapeutic Targets·Ming-Hua JiangJian-Guo Jiang
Nov 7, 2019·Food & Function·Fang-Chun Wu, Jian-Guo Jiang
Feb 12, 2021·International Journal of Biological Macromolecules·Yuan-Feng ZouChao Huang

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