PMID: 9159118May 27, 1997Paper

Activation of mitogen-activating protein kinase by glucose is not required for insulin secretion

Proceedings of the National Academy of Sciences of the United States of America
Shih Khoo, M H Cobb

Abstract

In the insulinoma cell line INS-1, a model system for glucose-regulated insulin secretion, the mitogen-activating protein (MAP) kinases/extracellular signal-regulated protein kinases, ERK1 and ERK2 are activated up to 15-fold by physiological concentrations of glucose, in the range of 3-12 mM. The related MAP kinase family members, the c-Jun-N-terminal kinases/stress-activated protein kinases are insensitive to glucose, while the p38 MAP kinase is slightly glucose responsive (1.5-fold). ERK activation is dependent on glucose metabolism and the subsequent increase in calcium influx. Inhibiting activation of ERK1 and ERK2 with the MEK1/2 inhibitor PD98059 has no effect on insulin secretion, indicating that ERK activity is not necessary for secretion under these conditions. Glucose activates ERK1 and ERK2 in cytosolic and purified nuclear fractions of INS-1 cells and more of each is found in nuclei from glucose-treated cells. These findings suggest that some of the glucose-dependent actions of ERKs will be exerted in the nucleus.

References

Mar 1, 1992·Molecular and Cellular Biology·R H ChenJ Blenis
Jan 1, 1989·Progress in Biophysics and Molecular Biology·F M Ashcroft, P Rorsman
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·A A ReszkaE H Fischer
Jan 1, 1995·Annual Review of Biochemistry·C B Newgard, J D McGarry
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·S XuM H Cobb
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
Jun 23, 1995·The Journal of Biological Chemistry·M H Cobb, E J Goldsmith
Dec 1, 1994·Trends in Biochemical Sciences·S EfratH F Lodish
May 12, 1994·Nature·J M KyriakisJ R Woodgett
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M S German
Apr 12, 1996·The Journal of Biological Chemistry·M ChengM H Cobb

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Citations

Oct 14, 2003·Biochemical Pharmacology·François RenaultHervé Chneiweiss
Jun 4, 1999·Progress in Biophysics and Molecular Biology·M H Cobb
Jul 10, 2003·Molecular and Cellular Endocrinology·Melissa K LingohrChristopher J Rhodes
Nov 13, 2001·Developmental Cell·E FormstecherH Chneiweiss
Mar 19, 2008·Cell Research·Michael C LawrenceMelanie H Cobb
Aug 24, 2013·Experimental & Molecular Medicine·Michael A Kalwat, Debbie C Thurmond
May 25, 2002·European Journal of Clinical Investigation·C J Rhodes, M F White
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michael C LawrenceMelanie H Cobb
Dec 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Lingling Duan, Melanie H Cobb
Nov 26, 2008·The Journal of Biological Chemistry·Chunli Shao, Melanie H Cobb
Aug 13, 2008·The Journal of General Physiology·Lavoisier S RamosLonny R Levin
Sep 27, 2007·Pancreas·Nikiforos BallianF Charles Brunicardi
Apr 25, 2007·Diabetes, Obesity & Metabolism·Kathrin Maedler
Jan 4, 2008·Acta Physiologica·M LawrenceM H Cobb
Nov 7, 2012·Molecular and Cellular Biology·Jung-Chun LinWoan-Yuh Tarn
Apr 4, 2006·Molecular and Cellular Biology·Gray W PearsonMelanie H Cobb
May 12, 2012·International Journal of Endocrinology·Elena TarabraMichael Khan
Feb 7, 2009·Lipids in Health and Disease·E-ri M SolPeter Bergsten
Jul 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michael C LawrenceMelanie H Cobb
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·G da Silva XavierG A Rutter
Feb 20, 2007·Pharmacology & Therapeutics·Máire E Doyle, Josephine M Egan
Apr 7, 2006·Molecular and Cellular Endocrinology·Gabriel F AnhêSilvana Bordin
May 11, 2007·Biochemical and Biophysical Research Communications·Sandra PiquerRamon Gomis
Nov 26, 2010·Islets·Gregory VlacichDonald F Steiner
Jun 30, 2009·Molecular Cell·Michele K DoughertyDeborah K Morrison
Jan 13, 2015·The Journal of Endocrinology·Xin-gang YaoXu Shen

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