Abstract
We show that osteopontin (OPN), bone sialoprotein (BSP) and GRGDSP peptides, in solution, induce activation of metalloproteinase-2 (MMP-2) secreted by human GCT23 giant cell tumour cells. Activation of MMP-2 is RGD sequence dependent, possibly involves anti-alphaVbeta3 integrins, is preceded by a change from spread to rounded cell morphology and is mimicked by the actin depolymerising agent cytochalasin B. Cells that had spread on OPN, BSP and GRGDSP substrata failed to activate MMP-2, but subsequent addition of soluble GRGDSP induced rounding and MMP-2 activation. Activation induced by GRGDSP and cytochalasin B was cell mediated, inhibited by EDTA, tissue inhibitor of metalloproteinase-2 (TIMP-2) and carboxyl terminal MMP-2 consistent with a role for membrane type (MT)-MMP but did not involve urokinase, plasmin or thrombin activity. Activation induced by GRGDSP and cytochalasin B, but not cell rounding, was inhibited by herbimycin A, cycloheximide and actinomycin D, suggesting a role for tyrosine kinases, protein and RNA synthesis, but was not associated with changes in mRNA for MT-MMP-1, MMP-1, MMP-2, TIMP-1 or TIMP-2. GRGDSP and cytochalasin B enhanced levels of membrane-associated pro- and active form MMP-1 and MMP-2 but no...Continue Reading
References
Sep 18, 1975·Nature·E D HarrisZ Werb
Jul 15, 1992·Biochemical and Biophysical Research Communications·A Reith, G J Rucklidge
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R E SeftorM J Hendrix
Mar 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M H HelfrichM A Horton
Aug 1, 1991·Experimental Cell Research·M A HortonM H Helfrich
Dec 27, 1990·European Journal of Biochemistry·Y OkadaH Nagase
Aug 1, 1989·The Journal of Cell Biology·Z WerbC H Damsky
Jun 1, 1989·The Anatomical Record·R Baron
Jan 1, 1989·Connective Tissue Research·W T Butler
Mar 1, 1986·The Journal of Cell Biology·Z WerbJ Aggeler
May 1, 1970·Cancer·D C DahlinE W Johnson
Jul 1, 1995·Endocrinology·K A HruskaD Cheresh
Apr 1, 1995·Journal of Cellular Biochemistry·J W XuanA F Chambers
Oct 6, 1995·The Journal of Biological Chemistry·S ZuckerW F Bahou
Nov 1, 1995·Clinical & Experimental Metastasis·V H RaoZ L Gokaslam
Feb 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M A HortonM H Helfrich
May 15, 1995·The Biochemical Journal·P A HillM C Meikle
Mar 10, 1995·The Journal of Biological Chemistry·A Y StronginG I Goldberg
Mar 17, 1995·The Journal of Biological Chemistry·R T Aimes, J P Quigley
Jul 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M GranoA Zambonin Zallone
Jul 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G van der PluijmC Löwik
Dec 6, 1994·Biochemistry·T CrabbeA J Docherty
Nov 15, 1994·The Biochemical Journal·R V WardG Murphy
Jul 7, 1994·Nature·H SatoM Seiki
Apr 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A L WucherpfennigP Stashenko
Feb 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G A Gronowicz, M E Derome
Jun 1, 1994·Experimental Cell Research·M GranoA Z Zallone
May 23, 1994·FEBS Letters·T CrabbeA Docherty
Nov 1, 1993·The American Journal of Physiology·R PanicciaA Teti
Jan 1, 1993·Kidney International·P D BrownW G Stetler-Stevenson
Dec 22, 1995·The Journal of Biological Chemistry·S J AtkinsonG Murphy
Feb 1, 1996·The Biochemical Journal·M Ailenberg, M Silverman
Jun 1, 1996·Endocrinology·M ChellaiahK A Hruska
May 31, 1996·Cell·P C BrooksD A Cheresh
Jul 15, 1996·European Journal of Biochemistry·J LohiJ Keski-Oja
Aug 1, 1996·Endocrine Reviews·G D Roodman
Oct 21, 1996·International Journal of Cancer. Journal International Du Cancer·C FestucciaA R Mackay
Dec 16, 1997·International Journal of Cancer. Journal International Du Cancer·A TiberioA R Mackay
Citations
Feb 23, 2008·Nature Reviews. Cancer·Akeila BellahcèneNeal S Fedarko
Sep 28, 2012·Beilstein Journal of Organic Chemistry·Grazia MaranoReinhard Schwartz-Albiez
Nov 18, 2005·Endothelium : Journal of Endothelial Cell Research·Margaret A HamnerMay J Reed
Jan 29, 2010·Diabetes·Florian W KieferThomas M Stulnig
Jan 13, 2006·Trends in Cell Biology·Hema RangaswamiGopal C Kundu
Mar 29, 2002·International Journal of Cancer. Journal International Du Cancer·Fumiyuki TakahashiKazuto Nishio
Jan 9, 2004·Oncogene·Yan-Nien LiuJ H Chen
Jan 6, 2004·The Journal of Biological Chemistry·Riku DasGopal C Kundu
Aug 6, 1999·Oncogene·A B TuckA F Chambers
May 20, 2004·British Journal of Cancer·V E MoyeP S Rudland
Feb 9, 2002·QJM : Monthly Journal of the Association of Physicians·M MazzaliJ Hughes
Jan 23, 2003·Microscopy Research and Technique·Noriharu ShijuboNoriyuki Sato
Aug 5, 2003·Journal of Cellular Biochemistry·Andrew I C SiMing H Zheng
Oct 19, 2011·Journal of Cellular Biochemistry·Stéphane BarthelemiSandrine Lorimier
Sep 28, 2004·Virchows Archiv : an International Journal of Pathology·M WuellingE Kaiser
Jun 28, 2001·Journal of Biomedical Materials Research·K WebbP A Tresco
Nov 25, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C FilantiP Manduca
Feb 24, 2005·British Journal of Haematology·Susanne VejdaThomas G Cotter
Jan 5, 2006·Brain Pathology·Dongyan WangCandece L Gladson
Jul 14, 2005·IUBMB Life·Riku DasGopal C Kundu
Jun 9, 2012·FEBS Letters·Antonietta R FarinaAndrew R Mackay
Jun 28, 2014·International Journal of Oncology·Shuxin ChenEnmin Li
Oct 4, 2003·Blood·Gerald W PragerBernd R Binder
Mar 1, 2007·Clinical Orthopaedics and Related Research·Michelle GhertGurmit Singh
Aug 26, 1999·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·T NagasakiS Otani
Dec 1, 2007·Endocrinology·Florian W KieferThomas M Stulnig
Oct 12, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S M Ellerbroek, M S Stack
Feb 23, 2007·Molecular Cancer Research : MCR·Thuy-Vy DoDavid A Fishman
Jun 6, 2007·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Lígia R RodriguesHelena Lindmark-Mänsson
Aug 25, 2012·Disease Models & Mechanisms·Rebecca A Mosig, John A Martignetti
Dec 9, 2000·Experimental Cell Research·E MaquoiJ M Foidart
Dec 13, 2000·Journal of Molecular and Cellular Cardiology·R BlindtJ vom Dahl
Oct 10, 2002·Experimental Cell Research·Claudio FestucciaMauro Bologna
Apr 25, 2006·Experimental Cell Research·V SamannaM A Chellaiah