Activation of mouse splenic lymphocyte guanylate cyclase by calcium ion

Journal of Biochemistry
T KatagiriT Osawa

Abstract

The guanosine 3',5'-cyclic monophosphate (cGMP) level in the mouse splenic lymphocytes was increased about 2- to 3-fold by concanavalin A. This increase was completely dependent on the presence of Ca2+ in the medium. Homogenates of mouse splenic lymphocytes contained significant guanylate cyclase [EC 4.6.1.2] activity in both the 105,000 X g (60 min) particulate and supernatant fractions and both fractions required Mn2+ for full activity. Calcium ion (3mM) activated soluble guanylate cyclase 3-fold at a relatively low concentration of Mn2+ (less than 1mM) but inhibited the particulate enzyme slightly at all Mn2+ concentrations tested. Concanavalin A itself did not stimulate either fraction of guanylate cyclase. Thus these results suggest that elevation of the cGMP level in lymphocytes by concanavalin A might be brought about by stimulation of Ca2+ uptake and activation of soluble guanylate cyclase by the latter.

Citations

May 1, 1987·Scandinavian Journal of Immunology·T E KnudsenH E Johnsen
Dec 2, 1976·Nature·S ToyoshimaT Osawa
Jan 1, 1979·Annals of the New York Academy of Sciences·J W HaddenE M Hadden
Jan 1, 1982·Journal of Immunopharmacology·T IkedaT Morimoto

Related Concepts

Metazoa
B-Lymphocytes
Calcium
Enzyme Activation
Guanylate Cyclase
Manganese
Mouse, Swiss

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