Activation of muscarinic acetylcholine receptors elicits pigment granule dispersion in retinal pigment epithelium isolated from bluegill

BMC Neuroscience
Alfredo GonzálezDana M García

Abstract

In fish, melanin pigment granules in the retinal pigment epithelium disperse into apical projections as part of the suite of responses the eye makes to bright light conditions. This pigment granule dispersion serves to reduce photobleaching and occurs in response to neurochemicals secreted by the retina. Previous work has shown that acetylcholine may be involved in inducing light-adaptive pigment dispersion. Acetylcholine receptors are of two main types, nicotinic and muscarinic. Muscarinic receptors are in the G-protein coupled receptor superfamily, and five different muscarinic receptors have been molecularly cloned in human. These receptors are coupled to adenylyl cyclase, calcium mobilization and ion channel activation. To determine the receptor pathway involved in eliciting pigment granule migration, we isolated retinal pigment epithelium from bluegill and subjected it to a battery of cholinergic agents. The general cholinergic agonist carbachol induces pigment granule dispersion in isolated retinal pigment epithelium. Carbachol-induced pigment granule dispersion is blocked by the muscarinic antagonist atropine, by the M1 antagonist pirenzepine, and by the M3 antagonist 4-DAMP. Pigment granule dispersion was also induced b...Continue Reading

References

Jan 1, 1992·Cell Motility and the Cytoskeleton·C D Thaler, L T Haimo
Jun 1, 1991·Journal of Neurochemistry·E L FeldmanD A Greene
Nov 1, 1990·The Journal of Cell Biology·C D Thaler, L T Haimo
Jan 1, 1989·Methods in Cell Biology·L T Haimo, M M Rozdzial
Mar 1, 1989·Experimental Eye Research·L L Troutt, B Burnside
Feb 1, 1994·The Journal of Cell Biology·K J Kotz, M A McNiven
Sep 1, 1995·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·S B Jones, L B King
Nov 30, 1999·Pigment Cell Research·M C Tuma, V I Gelfand
Dec 30, 1999·Progress in Retinal and Eye Research·N A Rao, G S Wu

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Citations

Jan 11, 2008·Clinical Journal of Sport Medicine : Official Journal of the Canadian Academy of Sport Medicine·Tanya M SmithJean-Michel Brismée
Aug 19, 2011·Journal of Receptor and Signal Transduction Research·Sharique A Ali, Keisham V Meitei
Sep 29, 2017·International Journal of Molecular Sciences·Kristin L Kopperud, Michael S Grace
Jul 12, 2011·Brain Research·Richard J NuckelsDana M García

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Methods Mentioned

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dissection

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