Activation of Na+/H+ exchange by protein phosphatase inhibitors in red blood cells of the frog Rana ridibunda

Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
Gennadii P Gusev, T I Ivanova

Abstract

The present study was designed to evaluate the role of protein phosphatases in regulation of sodium transport in the marsh frog erythrocytes using 22Na as a tracer. For this purpose the cells were treated with several known inhibitors of protein phosphatases. In standard isotonic medium, exposure of the cells to 10 mmol l(-1) NaF, 20 nmol l(-1) calyculin A or 0.1 mmol l(-1) cantharidin resulted in a significant (1.7-fold) increase in unidirectional ouabain-insensitive Na+ influx. The Na+ influx in frog red cells was progressively activated as the medium osmolality was increased by addition of 100, 200 or 300 mmol l(-1) sucrose to standard isotonic medium. The stimulatory effect of protein phosphatase blockers on Na+ influx was much higher in hypertonic medium containing 100 or 200 mmol l(-1) sucrose than that in isotonic medium. Stimulation of Na+ transport enhanced with increasing concentrations of calyculin A, and half-maximal activation (EC50) was obtained at 16 nmol l(-1). However, Na+ influx induced by strong hypertonic treatment (+300 mmol l(-1) sucrose) was not altered further in the presence of protein phosphatase inhibitors. The changes in Na+ influx evoked by protein phosphatase inhibitors and hypertonic treatment wer...Continue Reading

Citations

Jul 1, 2006·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·G P Gusev, T I Ivanova
May 7, 2008·Zoology : Analysis of Complex Systems, ZACS·Laura N Cruz, Marta M Souza
Aug 1, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Tatiana I IvanovaGennadii P Gusev
Jun 19, 2007·The Journal of Experimental Biology·Karina KristensenTobias Wang

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