Activation of nervous system development genes in bone marrow derived mesenchymal stem cells following spaceflight exposure

Journal of Cellular Biochemistry
Massimiliano MonticoneRanieri Cancedda

Abstract

Stalled cell division in precursor bone cells and reduced osteoblast function are considered responsible for the microgravity-induced bone loss observed during spaceflight. However, underlying molecular mechanisms remain unraveled. Having overcome technological difficulties associated with flying cells in a space mission, we present the first report on the behavior of the potentially osteogenic murine bone marrow stromal cells (BMSC) in a 3D culture system, flown inside the KUBIK aboard space mission ISS 12S (Soyuz TMA-8 + Increment 13) from March 30 to April 8, 2006 (experiment "Stroma-2"). Flight 1g control cultures were performed in a centrifuge located within the payload. Ground controls were maintained on Earth in another KUBIK payload and in Petri dishes. Half of the cultures were stimulated with osteo-inductive medium. Differences in total RNA extracted suggested that cell proliferation was inhibited in flight samples. Affymetrix technology revealed that 1,599 genes changed expression after spaceflight exposure. A decreased expression of cell-cycle genes confirmed the inhibition of cell proliferation in space. Unexpectedly, most of the modulated expression was found in genes related to various processes of neural develop...Continue Reading

References

Jun 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K C Westerlind, R T Turner
Mar 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R RabinF M Sulzman
Oct 1, 1996·Medicine and Science in Sports and Exercise·K M BaldwinC M Snow
May 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G CarmelietR Bouillon
Jun 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G Carmeliet, R Bouillon
Apr 13, 2001·Bioinformatics·K Y YeungW L Ruzzo
Oct 1, 1995·Acta Astronautica·V SchneiderL Varonin
Mar 5, 2003·BioTechniques·A I SaeedJ Quackenbush
Oct 2, 2003·Genome Biology·Douglas A HosackRichard A Lempicki
Feb 18, 2004·Annals of Biomedical Engineering·Anna R Derubeis, Ranieri Cancedda
Nov 24, 2004·Differentiation; Research in Biological Diversity·Tatiana TondreauDominique Bron
Feb 16, 2006·The International Journal of Developmental Biology·Susan J Crawford-Young
Apr 7, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yan WangGang Li
Jul 28, 2007·Annals of the New York Academy of Sciences·Ritu SaxenaJay M McDonald
Aug 16, 2008·Experimental Brain Research·Antonio FrigeriDavid C Spray
Mar 19, 2009·Tissue Engineering. Part a·Federico TortelliRanieri Cancedda

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Citations

May 10, 2013·Bulletin of Experimental Biology and Medicine·M Yu KapitonovaH Nawawi
Apr 29, 2014·Bulletin of Experimental Biology and Medicine·M Yu KapitonovaG R A Froemming
Dec 3, 2014·Stem Cells and Development·Elizabeth BlaberEduardo A C Almeida
Nov 6, 2014·Life·Silvia BradamanteJeanette A M Maier
Mar 26, 2015·Preparative Biochemistry & Biotechnology·Claudia KochJohann Bauer
Sep 29, 2015·Stem Cells and Development·Elizabeth A BlaberEduardo A C Almeida
Oct 3, 2014·BioMed Research International·Maria Elena PisanuRita Mancini
Mar 19, 2015·BioMed Research International·Giada Graziana GenchiGianni Ciofani
Sep 27, 2016·Progress in Biophysics and Molecular Biology·Xinchang ZhangXizheng Zhang
Sep 28, 2017·International Journal of Molecular Sciences·Elizabeth A BlaberKaren R Jonscher
Jul 7, 2018·Stem Cells and Development·Takeshi ImuraLouis Yuge
Jan 10, 2019·Journal of Cellular Physiology·Qinyu MaShiwu Dong
Feb 6, 2021·Life·Macauley J GreenPhilip M Williams
Apr 28, 2021·Journal of Environmental Science and Health. Part C, Toxicology and Carcinogenesis·Jeffrey S WilleyXiao Wen Mao
May 20, 2021·Stem Cells International·Uğur Topal, Cihan Zamur

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