Activation of neuronal N-methyl-D-aspartate receptor plays a pivotal role in Japanese encephalitis virus-induced neuronal cell damage

Journal of Neuroinflammation
Zheng ChenShengbo Cao

Abstract

Overstimulation of glutamate receptors, especially neuronal N-methyl-D-aspartate receptor (NMDAR), mediates excitatory neurotoxicity in multiple neurodegenerative diseases. However, the role of NMDAR in the regulation of Japanese encephalitis virus (JEV)-mediated neuropathogenesis remains undisclosed. The primary objective of this study was to understand the function of NMDAR to JEV-induced neuronal cell damage and inflammation in the central nervous system. The effect of JEV-induced NMDAR activation on the progression of Japanese encephalitis was evaluated using the primary mouse neuron/glia cultures and a mouse model of JEV infection. A high-affinity NMDAR antagonist MK-801 was employed to block the activity of NMDAR both in vitro and in vivo. The subsequent impact of NMDAR blockade was assessed by examining the neuronal cell death, glutamate and inflammatory cytokine production, and JEV-induced mice mortality. JEV infection enhanced the activity of NMDAR which eventually led to increased neuronal cell damage. The data obtained from our in vitro and in vivo assays demonstrated that NMDAR blockade significantly abrogated the neuronal cell death and inflammatory response triggered by JEV infection. Moreover, administration of N...Continue Reading

References

Nov 1, 1992·Journal of Neurobiology·D W Choi
Jan 1, 1984·Journal of Neurochemistry·F Fonnum
Oct 1, 1995·Trends in Pharmacological Sciences·I Mody, J F MacDonald
Jan 1, 1994·Progress in Brain Research·D W Choi
Sep 21, 2004·Journal of Bioenergetics and Biomembranes·Valina L Dawson, Ted M Dawson
Nov 13, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hee Jung ChungRichard L Huganir
Jul 24, 2007·Neuropharmacology·Bo-Shiun Chen, Katherine W Roche
Aug 20, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F LéveilléA Buisson
Sep 30, 2009·PLoS Neglected Tropical Diseases·Debapriya Ghosh, Anirban Basu
Dec 17, 2009·The Journal of General Virology·Chun-Jung ChenJian-Hong Chen
Feb 6, 2010·Progress in Neurobiology·Usha Kant Misra, Jayantee Kalita
Aug 19, 2010·Pharmacological Reviews·Stephen F TraynelisRay Dingledine
Oct 26, 2011·Brain Research Bulletin·Joshua A SmithNaren L Banik
Feb 14, 2012·Current Opinion in Neurobiology·David L Hunt, Pablo E Castillo
Dec 12, 2012·International Journal of Molecular Sciences·Jefree JohariKeivan Zandi
Apr 16, 2013·Neuropharmacology·Qiang Zhou, Morgan Sheng
May 21, 2013·Nature Reviews. Neuroscience·Pierre PaolettiQiang Zhou
Sep 21, 2013·CNS & Neurological Disorders Drug Targets·Madhuvika MuruganCharanjit Kaur
Oct 29, 2013·Human Vaccines & Immunotherapeutics·Sang-Im Yun, Young-Min Lee
Dec 3, 2013·European Journal of Pharmacology·John Q WangLi-Min Mao
Mar 22, 2014·The Journal of Infectious Diseases·Jing YeShengbo Cao
Aug 8, 2014·PLoS Neglected Tropical Diseases·Khin Saw Aye MyintTom Solomon
Feb 25, 2015·Neuropsychiatric Disease and Treatment·Aysegul Yildiz-UnalArzu Karabay
Jul 24, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bibo ZhuShengbo Cao
May 18, 2016·The Journal of Comparative Neurology·Christopher S von BartheldSuzana Herculano-Houzel
Aug 23, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yisong QianHongbin Sun
Feb 18, 2017·Journal of Alzheimer's Disease : JAD·Ramesh Kandimalla, P Hemachandra Reddy
Mar 16, 2017·Frontiers in Microbiology·Peiyu BianYingfeng Lei

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
ELISA
PCR
Protein Assay
flow cytometry

Software Mentioned

GraphPad
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.