Activation of neuronal SST₁ and SST₂ receptors decreases neurogenic secretion in the guinea-pig jejunum

Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society
Jaime Pei Pei FoongJ C Bornstein

Abstract

Vasoactive intestinal peptide (VIP) submucosal neurons, the main regulators of gut secretion, display inhibitory postsynaptic potentials mediated by somatostatin (SOM) acting on SST(1) and SST(2) receptors (SSTR(1), SSTR(2)) in the guinea-pig small intestine. We investigated the implications of this for neurally-evoked mucosal secretion. Mucosal-submucosal preparations from guinea-pig jejunum were mounted in Ussing chambers to measure Cl(-) secretion, measured by short circuit current (I(sc)). All drugs were added serosally. Veratridine (1 μmol L(-1)) was used to stimulate neurons and provide a robust secretory response for pharmacological testing.5-hydroxytrptamine (5-HT, 300 nmol L(-1)) was used to specifically activate non-cholinergic secretomotor neurons, while 1,1-dimethyl-4-phenylpiperazinium (DMPP, 10 μmol L(-1)) was used to stimulate all secretomotor neurons. Somatostatin (50 nmol L(-1)) induced a tetrodotoxin (TTX, 1 μmol L(-1))-sensitive decrease in secretion. Somatostatin also reduced the veratridine-induced increase in I(sc). The effects of SOM were significantly reduced by blocking SSTR(1) and SSTR(2) individually or together. Blocking SSTR(1) abolished the inhibition produced by SOM. Quantitative PCR demonstrated ...Continue Reading

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Citations

Oct 18, 2011·Digestive Diseases and Sciences·Michael CamilleriTyler Nelsen
Dec 18, 2013·The Journal of Physiology·Jaime Pei Pei FoongJoel C Bornstein
Jun 14, 2018·Physiology·Nathalie Vergnolle, Carla Cirillo
Mar 1, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Candice FungJaime P P Foong

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