Activation of NFkappaB represents the central event in the neoplastic progression associated with Barrett's esophagus: a possible link to the inflammation and overexpression of COX-2, PPARgamma and growth factors

Digestive Diseases and Sciences
Peter C KonturekSteffen Mühldorfer

Abstract

The molecular mechanisms responsible for the progression of malignant transformation in Barrett's esophagus (BE) are still poorly understood. This study was undertaken (1) to investigate the gene and protein expression of cyclooxygenase-2 (COX-2), peroxisome proliferator-activated receptor-gamma (PPARgamma), interleukin-8 (IL-8), hepatocyte growth factor (HGF), gastrin, and its receptor (CCK-2) in the Barrett's epithelium; (2) to analyze the activity of NFkappaB in Barrett's esophagus with low-grade dysplasia; and (3) to assess the effects of PPARgamma ligand (ciglitazone) and gastrin on cell proliferation in the cell line derived from esophageal adenocarcinoma (OE-33). COX-2, PPARgamma, IL-8, HGF, gastrin, and CCK-2 expression levels relative to the control gene encoding GAPDH were analyzed by RT-PCR and Western blot in specimens of BE with low-grade dysplasia (n = 20) and compared with that in the normal squamous esophageal mucosa from the middle portion of the esophagus (n = 20). In vitro experiments included the incubation of cell line OE-33 with ciglitazone (1-15 microM) and gastrin (100 nM). NFkappaB activity in biopsies specimens was measured by highly sensitive ELISA. COX-2, PPARgamma, IL-8, HGF, gastrin, and CCK-2 expr...Continue Reading

Citations

Nov 28, 2013·Archivum Immunologiae Et Therapiae Experimentalis·Shegan GaoXiaoshan Feng
Oct 11, 2011·Digestive Diseases and Sciences·Hao ChenXiaoxin Chen
Dec 10, 2008·Journal of Gastrointestinal Cancer·Sumera IlyasNavtej S Buttar
Apr 10, 2012·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·N RawatG J S Jenkins
Apr 26, 2012·Journal of Chemical Information and Modeling·Amit K GuptaAnil K Saxena
Jan 19, 2010·Gastroenterology·Stuart Jon SpechlerKenneth K Wang
Nov 16, 2007·Nutrition and Cancer·Naihsuan C GuyKaterina Dvorak
Oct 5, 2010·PPAR Research·Yuji NaitoToshikazu Yoshikawa
Feb 22, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Liying YangZhiheng Pei
Oct 13, 2011·World Journal of Gastroenterology : WJG·Elizabeth F Wiseman, Yeng S Ang
Dec 14, 2012·World Journal of Gastroenterology : WJG·Eduardo ChuecaElena Piazuelo
Oct 25, 2006·Alimentary Pharmacology & Therapeutics·S MehtaM Rhodes
Jan 26, 2006·Basic & Clinical Pharmacology & Toxicology·Robert T Jensen
Nov 15, 2011·Biochimica Et Biophysica Acta·Hannelien VerbekeStruyf Sofie
May 1, 2012·Pathology, Research and Practice·A PoehlmannA Roessner
Jul 15, 2009·Current Opinion in Pharmacology·Mohamed M M Abdel-LatifDermot Kelleher
Jan 13, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Julie G IzzoJaffer A Ajani
Apr 12, 2017·Molecular and Cellular Biochemistry·Arumugam MadankumarThiruvengadam Devaki
Nov 22, 2007·Bioorganic & Medicinal Chemistry Letters·Craig R WoodsMichael H Rabinowitz
Mar 13, 2016·Seminars in Oncology·Antonio Galvao NetoZhiheng Pei
Feb 1, 2007·ORL; Journal for Oto-rhino-laryngology and Its Related Specialties·Konstantinos KourelisPanagiotis Goumas
Jan 29, 2008·International Journal of Cancer. Journal International Du Cancer·Rodrigo BarderasJosé Ignacio Casal
Nov 26, 2009·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·M BabarJ V Reynolds
Aug 12, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Matthias RehnMartin Diener
Aug 23, 2005·Carcinogenesis·Shane P DugganDermot Kelleher
Jul 4, 2006·Physiological Reviews·Marlène DufresneDaniel Fourmy
Jul 11, 2019·Pathology International·Ken-Ichi MukaishoHiroyuki Sugihara
Jan 14, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jolanta MajkaTomasz Brzozowski
Apr 30, 2017·Oncotarget·Chenli YueXinhan Zhao
Apr 10, 2014·International Journal of Experimental Pathology·Nicola H GreenJonathan P Bury
Sep 12, 2014·Journal of Basic and Clinical Physiology and Pharmacology·Venkatachalam KarthikkumarNamasivayam Nalini
Feb 7, 2013·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·X-J TengS-S Xiao
Mar 26, 2015·World Journal of Gastroenterology : WJG·Sahil GambhirArpita Vyas
Jun 5, 2018·Annals of Gastroenterological Surgery·Yoshifumi BabaHideo Baba
Sep 1, 2019·Drug Development Research·Vijay L KumarPrasenjit Das

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