PMID: 9450943Mar 7, 1998Paper

Activation of nicotinic acetylcholine receptors augments calcium channel-mediated exocytosis in rat pheochromocytoma (PC12) cells

The Journal of General Physiology
Amy B Harkins, A P Fox

Abstract

The functional effect of activating Ca2+-permeable neuronal nicotinic acetylcholine receptors (nAChRs) on vesicle secretion was studied in PC12 cells. Single cells were patch-clamped in the whole-cell configuration and stimulated with either brief pulses of nicotine to activate the Ca2+-permeable nAChRs or with voltage steps to activate voltage-dependent Ca2+ channels. Membrane capacitance was used as a measure of vesicle secretion. Activation of nAChRs by nicotine application to cells voltage clamped at -80 mV evoked secretion. This secretion was completely abolished by nicotinic antagonists. When the cells were voltage clamped at +20 mV in the presence of Cd2+ to block voltage-activated Ca2+ channels, nicotine elicited a small amount of secretion. Most interestingly, when the nAChRs were activated coincidentally with voltage-dependent Ca2+ channels, secretion was augmented approximately twofold over the secretion elicited with voltage-dependent Ca2+ channels alone. Our data suggest that Ca2+ influx via nAChRs affects Ca2+-dependent cellular functions, including vesicle secretion. In addition to the secretion evoked by nAChR activation at hyperpolarized potentials, we demonstrate that even at depolarized potentials, nAChRs pro...Continue Reading

References

Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D E Burstein, L A Greene
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Nov 1, 1992·The Journal of Physiology·C K Ifune, J H Steinbach
Mar 1, 1992·Trends in Pharmacological Sciences·B P Bean
May 1, 1991·The Journal of Physiology·L A Wong, J P Gallagher
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jan 1, 1983·The Journal of Membrane Biology·R Latorre, C Miller
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E Neher, A Marty
Dec 1, 1993·Pflügers Archiv : European journal of physiology·Z Zhou, E Neher
Jan 1, 1993·Annual Review of Neuroscience·P B Sargent
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·P MollardM C Nowycky
Feb 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A C Thomas-Reetz, P De Camilli

❮ Previous
Next ❯

Citations

Mar 17, 2004·Archives of Biochemistry and Biophysics·O L ZaikaE A Lukyanetz
Jul 29, 2010·American Journal of Physiology. Cell Physiology·Dangjai SouvannakittiNanduri R Prabhakar
Feb 5, 2014·Toxicon : Official Journal of the International Society on Toxinology·Tomohiko KayanoTadanori Aimi
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y J Cao, Y Y Peng
Oct 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Amy B HarkinsAaron P Fox
Nov 6, 2007·Biochemical and Biophysical Research Communications·Juan J Arnáiz-CotAntonio G García
Nov 25, 2003·Alcoholism, Clinical and Experimental Research·Douglas P Dohrman And, Cindy K Reiter
Jul 15, 2017·ACS Chemical Neuroscience·Brooke A MillerAmy B Harkins
Jul 18, 2003·Journal of Neurophysiology·Amy B HarkinsAaron P Fox
Feb 13, 2009·American Journal of Physiology. Cell Physiology·Laura TapiaLuis Gandía

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.