Activation of Nippostrongylus brasiliensis infective larvae is regulated by a pathway distinct from the hookworm Ancylostoma caninum

International Journal for Parasitology
Stanley Ching-Cheng HuangMurray E Selkirk

Abstract

Developmentally arrested infective larvae of strongylid nematodes are activated to resume growth by host-derived cues encountered during invasion of the mammalian host. Exposure of Nippostrongylus brasiliensis infective larvae to elevated temperature (37°C) is sufficient to activate signalling pathways which result in resumption of feeding and protein secretion. This occurs independently of exposure to serum or glutathione, in contrast to the hookworm Ancylostoma caninum, and is not initiated by chemical exsheathment. No qualitative differences in protein secretion were induced by host serum as visualised by two-dimensional SDS-PAGE, although exposure of larvae to an aqueous extract of rat skin did stimulate secretion of a small pre-synthesised bolus of proteins. Infective larvae began feeding after a lag period of 3-4 h at 37°C, reaching a maximum of 90% of the population feeding by 48 h. Neither a membrane permeant analogue of cyclic GMP nor muscarinic acetylcholine receptor agonists stimulated feeding at 20°C, and high concentrations of both compounds inhibited temperature-induced activation. LY294002, an inhibitor of phosphatidylinositol 3-kinase, Akt inhibitor IV, an inhibitor of Akt protein kinase, and ketoconazole, an in...Continue Reading

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Citations

Jan 6, 2012·Parasitology·Johnathan J DalzellAaron G Maule
Mar 31, 2012·Parasitology·Murray E SelkirkCollette Britton
Dec 21, 2012·Parasitology·Juan E Palomares-RiusVivian C Blok
Feb 5, 2016·Parasites & Vectors·Namitha MohandasRobin B Gasser
Dec 1, 2011·International Journal for Parasitology. Drugs and Drug Resistance·Adrian J Wolstenholme
Oct 8, 2013·International Journal for Parasitology·Matt Crook
Apr 7, 2017·Journal of Neurochemistry·Arnaud ChatonnetMurray E Selkirk
Jul 12, 2020·Proceedings of the National Academy of Sciences of the United States of America·Spencer S GangElissa A Hallem

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