Activation of NLRP3 inflammasome enhances the proliferation and migration of A549 lung cancer cells

Oncology Reports
Yanli WangWeiping Xie

Abstract

Lung cancer is the leading cause of cancer death, and it is widely accepted that chronic inflammation is an important risk for the development of lung cancer. Now, it is recognized that the nucleotide-binding and oligomerization domain (NOD) like receptors (NLRs)-containing inflammasomes are involved in cancer-related inflammation. This study was designed to investigate the effects of NLR family pyrin domain containing protein 3 (NLRP3) inflammasome on the proliferation and migration of lung adenocarcinoma cell line A549. Using 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay, scratch assay, and Transwell migration assay, we showed that activation of the NLRP3 inflammasome by LPS+ATP enhanced the proliferation and migration of A549 cells. Western blot analysis showed that activation of phosphorylation of Akt, ERK1/2, CREB and the expression of Snail increased, while the expression of E-cadherin decreased after the activation of NLRP3 inflammasome. Moreover, these effects were inhibited by the following treatments: i) downregulating the expression of NLRP3 by short hairpin RNA (shRNA) interference, ii) inhibiting the activation of NLRP3 inflammasome with a caspase-1 inhibitor, iii) blocking the interleukin-1β (IL-1β) and IL-1...Continue Reading

References

Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·F Vidal-VanaclochaC A Dinarello
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yasuo SaijoToshihiro Nukiwa
Dec 20, 2002·Nature·Lisa M Coussens, Zena Werb
May 3, 2006·Biochemical and Biophysical Research Communications·Jihye KimDaeho Cho
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Mona Johannessen, Ugo Moens
Aug 30, 2008·The Journal of Experimental Medicine·Jonathan MaelfaitRudi Beyaert
Sep 11, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carl F FortinPatrick P McDonald
Sep 16, 2008·The Journal of Dermatology·Yuka HosotaniHaruki Okamura
Jan 6, 2009·Nature·Alberto Mantovani
Mar 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kyung-Eun KimDae-Ho Cho
Jun 30, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Balachandar VenkatesanBysani Chandrasekar
Sep 23, 2009·Cell Cycle·Yadi Wu, Binhua P Zhou
Apr 14, 2010·The Journal of Experimental Medicine·Irving C AllenJenny P-Y Ting
Feb 16, 2011·Journal of Cellular Physiology·Venkatapuram Seenu ReddyBysani Chandrasekar
Mar 15, 2011·Cytokine·Nikoletta RovinaPetros Bakakos
May 31, 2011·Cancer Biology & Therapy·Zhaowei ZhuZhoujun Shen
Dec 2, 2011·Cytokine·Obul R BandapalliKarsten Brand
Jan 20, 2012·Nature·Till StrowigRichard Flavell
Mar 21, 2012·Nature Immunology·Laurence ZitvogelGuido Kroemer
May 7, 2013·Cell Death and Differentiation·V SagulenkoK J Stacey
Jul 23, 2013·Progress in Neuro-psychopharmacology & Biological Psychiatry·Kui Y YangDong K Ahn
Sep 10, 2013·Molecular Neurobiology·Xian-Fang MengChun Zhang
Oct 1, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christina AntonopoulosGeorge R Dubyak
Nov 26, 2013·Frontiers in Immunology·Ann M JanowskiFayyaz S Sutterwala
Jan 9, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Jan 30, 2014·Protein & Cell·Ryan KolbWeizhou Zhang
Feb 27, 2014·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Adel HelmyPeter J Hutchinson
Jun 2, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mousumi TaniaJunjiang Fu
Jun 6, 2014·Journal of Immunology Research·Glauben LandskronMarcela A Hermoso
Jul 24, 2014·Journal of Cellular Biochemistry·María Julia MartínClaudia Gentili
Sep 11, 2014·Journal of Cellular Physiology·Chia-Huei LeeKo-Jiunn Liu
Dec 31, 2014·Journal of Leukocyte Biology·Marina FabbiSilvano Ferrini

❮ Previous
Next ❯

Citations

Sep 4, 2017·Journal of Experimental & Clinical Cancer Research : CR·Cong-Fa HuangZhi-Jun Sun
Oct 27, 2017·European Respiratory Review : an Official Journal of the European Respiratory Society·Francois Ng Kee KwongKian Fan Chung
Jun 24, 2017·Journal of Experimental & Clinical Cancer Research : CR·Xiaodong FengFuxiang Chen
Apr 7, 2019·Journal of Cellular Physiology·Minda ZhangRong Hu
Mar 7, 2020·Frontiers in Oncology·Huiyan ChengHuanfa Yi
Jul 2, 2020·Cells·Maria Sebastian-Valverde, Giulio M Pasinetti
Oct 6, 2020·Frontiers in Oncology·Margherita SonnessaAnita Mangia
Feb 17, 2019·Cell Death & Disease·KeShan WangXiaoPing Zhang
Jun 25, 2020·BMC Complementary Medicine and Therapies·Xuan WangZu-Jun Sun
Aug 1, 2020·Frontiers in Immunology·Shaima'a Hamarsheh, Robert Zeiser
Feb 14, 2017·Annual Review of Physiology·Judie A Howrylak, Kiichi Nakahira
Sep 16, 2018·Cellular and Molecular Neurobiology·Sen ShangXiaoyun Lu
Mar 8, 2019·Nature Reviews. Cancer·Rajendra Karki, Thirumala-Devi Kanneganti
Nov 19, 2018·Molecular Cancer·Maryam MoossaviAmirhossein Sahebkar
Sep 16, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wei ZhaoJohn H Kehrl
Jan 5, 2021·Chinese Medical Journal·Feng ZhouSong-Ying Zhang
Nov 24, 2019·Pathology, Research and Practice·Bin WangXinguo Zhu
Feb 4, 2021·Archives of Pharmacal Research·Jin Kyung SeokJoo Young Lee
Dec 22, 2020·Plastic and Reconstructive Surgery·Francesca FerraraGiuseppe Valacchi
Feb 23, 2021·Frontiers in Immunology·Ting-Yi LinChia-Yang Li
May 9, 2021·Pediatric Research·Tayfun Hilmi AkbabaBanu Balci-Peynircioglu
Nov 5, 2020·Cytokine & Growth Factor Reviews·Carla FourieAnna-Mart Engelbrecht
May 12, 2021·Biochemical and Biophysical Research Communications·Yuhao WangFei Zou
May 27, 2021·Environmental Toxicology·Mengyuan LiFeifei Feng
Jul 3, 2021·Frontiers in Immunology·Chaoqing ZhongDaoxin Ma
Jul 10, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Ya-Xin LvDan-Dan Zhang
Jul 25, 2021·Cancers·Xiuxia LuXing Zhang
Feb 21, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuan YangXiaoguang Zhou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.