Activation of P2X7 receptors decreases the proliferation of murine luteal cells

Reproduction, Fertility, and Development
Jing WangChunping Zhang

Abstract

Extracellular ATP regulates cellular function in an autocrine or paracrine manner through activating purinergic signalling. Studies have shown that purinergic receptors were expressed in mammalian ovaries and they have been proposed as an intra-ovarian regulatory mechanism. P2X7 was expressed in porcine ovarian theca cells and murine and human ovarian surface epithelium and is involved in ATP-induced apoptotic cell death. However, the role of P2X7 in corpus luteum is still unclear. The aim of this study was to investigate the role of ATP signalling in murine luteal cells and the possible mechanism(s) involved. We found that P2X7 was highly expressed in murine small luteal cells. The agonists of P2X7, ATP and BzATP, inhibited the proliferation of luteal cells. P2X7 antagonist BBG reversed the inhibition induced by ATP and BzATP. Further studies showed that ATP and BzATP inhibited the expression of cell cycle regulators cyclinD2 and cyclinE2. ATP and BzATP also inhibited the p38-mitogen-activated protein kinase (MAPK) signalling pathway. These results reveal that P2X7 receptor activation is involved in corpus luteum formation and function.

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Citations

Mar 17, 2018·BioMed Research International·Zhenhua JiYongping You
Mar 22, 2018·Oncotarget·Ponarulselvam SekarWan-Wan Lin
Aug 15, 2015·Molecular Reproduction and Development·Angélica S Martínez-Ramírez, Francisco G Vázquez-Cuevas
Jan 1, 2018·Purinergic Signalling·Karin DreisigBirgitte Rahbek Kornum

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
reverse transcription PCR
electrophoresis

Software Mentioned

SPSS
BOSTER

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis