Activation of P2X7 Receptors in Peritoneal and Meningeal Mast Cells Detected by Uptake of Organic Dyes: Possible Purinergic Triggers of Neuroinflammation in Meninges

Frontiers in Cellular Neuroscience
Dilyara NurkhametovaRashid Giniatullin

Abstract

Extracellular ATP activates inflammasome and triggers the release of multiple cytokines in various immune cells, a process primarily mediated by P2X7 receptors. However, the expression and functional properties of P2X7 receptors in native mast cells in tissues such as meninges where migraine pain originates from have not been explored. Here we report a novel model of murine cultured meningeal mast cells and using these, as well as easily accessible peritoneal mast cells, studied the mechanisms of ATP-mediated mast cell activation. We show that ATP induced a time and dose-dependent activation of peritoneal mast cells as analyzed by the uptake of organic dye YO-PRO1 as well as 4,6-diamidino-2-phenylindole (DAPI). Both YO-PRO1 and DAPI uptake in mast cells was mediated by the P2X7 subtype of ATP receptors as demonstrated by the inhibitory effect of P2X7 antagonist A839977. Consistent with this, significant YO-PRO1 uptake was promoted by the P2X7 agonist 2',3'-O-(benzoyl-4-benzoyl)-ATP (BzATP). Extracellular ATP-induced degranulation of native and cultured meningeal mast cells was shown with Toluidine Blue staining. Taken together, these data demonstrate the important contribution of P2X7 receptors to ATP-driven activation of mast ...Continue Reading

References

Jun 27, 1981·Lancet·G Burnstock
Feb 28, 1997·The Journal of Biological Chemistry·F RassendrenA Surprenant
Dec 16, 1998·FEBS Letters·I P ChessellP P Humphrey
Feb 27, 1999·Naunyn-Schmiedeberg's Archives of Pharmacology·A D MichelP P Humphrey
Aug 10, 1999·European Journal of Pharmacology·B R BianchiT van Biesen
Sep 14, 1999·British Journal of Pharmacology·C B GrahamesP P Humphrey
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·R A North, A Surprenant
Apr 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Petra J G ZwijnenburgA Marceline Van Furth
Oct 9, 1951·Acta Physiologica Scandinavica·R HARMA, P SUOMALAINEN
Aug 12, 2005·American Journal of Physiology. Cell Physiology·Lin-Hua JiangR Alan North
Mar 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Davide FerrariFrancesco Di Virgilio
Dec 19, 2006·Cellular Signalling·Samantha F Moore, Amanda B MacKenzie
Jul 14, 2007·The Journal of Biological Chemistry·Tina PangršičRobert Zorec
Apr 25, 2008·Current Protocols in Immunology·Bettina M JensenAlasdair M Gilfillan
May 22, 2009·Current Pain and Headache Reports·Dan Levy
Feb 19, 2011·Nature Reviews. Immunology·Wolfgang G Junger
May 9, 2012·Nature Medicine·Stephen J Galli, Mindy Tsai
Jun 1, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michela FrascoliFabio Grassi
Jun 9, 2012·Frontiers in Immunology·Axel LorentzUlrich Blank
Sep 6, 2012·Nature Communications·Yosuke KurashimaHiroshi Kiyono
Feb 22, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Liam E BrowneR Alan North
Sep 13, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Liam E Browne, R Alan North
Dec 18, 2013·Pharmacological Reviews·Geoffrey Burnstock, Vera Ralevic
Jun 7, 2014·Nature Reviews. Immunology·Sara Wernersson, Gunnar Pejler
Sep 17, 2014·Trends in Pharmacological Sciences·Beáta Sperlágh, Peter Illes
Oct 30, 2014·Purinergic Signalling·Geoffrey Burnstock, Jean-Marie Boeynaems
Dec 3, 2014·Frontiers in Immunology·Tae Chul MoonMarianna Kulka
Feb 19, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alessia FranceschiniFrancesco Di Virgilio
Mar 10, 2015·Stem Cells and Development·Joakim S DahlinJenny Hallgren
May 27, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Gerhard Dahl
Jun 5, 2015·Frontiers in Immunology·Björn RissiekSahil Adriouch
Jun 8, 2015·Current Pain and Headache Reports·Dagny Holle, Mark Obermann

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Citations

Sep 29, 2020·Frontiers in Cellular Neuroscience·Kseniia KorolevaGuzel Sitdikova
Apr 27, 2019·Frontiers in Cellular Neuroscience·Duygu Koyuncu IrmakFatma Tore
Nov 27, 2020·Journal of Neuroinflammation·Huaping XuLinbo Shi
Dec 18, 2020·Frontiers in Cellular Neuroscience·Dilyara NurkhametovaRashid Giniatullin
Mar 23, 2021·Annals of Neurology·Amanda AvonaGregory Dussor
Aug 1, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Haruhisa NishiEdward S Schulman
Nov 21, 2021·Purinergic Signalling·Wen-Jing Ren, Peter Illes

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Methods Mentioned

BETA
flow cytometry
lavage
scraping
light scattering

Software Mentioned

Kaluza
Statistica
GraphPad Prism
Origin
CytExpert

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