Abstract
Artocarpol A (ART), a natural product isolated from Artocarpus rigida, stimulated superoxide anion (O2*-) generation, which was inhibited by 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY 294002), a phosphoinositide 3-kinase (PI3K) inhibitor, in rat neutrophils. ART stimulated phosphorylation of protein kinase B (PKB/Akt) on both T308 and S473 residues, and LY 294002 inhibited these effects. Rat neutrophils expressed both class IA PI3K subunits (p85, p110alpha, p110beta, and p110delta) and a class IB PI3K subunit (p110gamma) as assessed by a combination of Western blotting and reverse transcription-polymerase chain reaction (RT-PCR) approaches. Stimulation of neutrophils with ART evoked phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) formation, which reached a maximal level at 2 min and was attenuated by LY 294002, as evidenced by immunofluorescence microscopy and by flow cytometry. Detectable membrane-association of class IA PI3Ks, class IB PI3K and Ras was seen as early as 1.5, 0.5 and 1.5 min, respectively, after stimulation with ART. The kinetics of ART-induced Ras activation paralleled the kinetics of class IA PI3Ks recruitment to membrane caused by ART, and the p85 and p110gamma immunoprecipitates contain...Continue Reading
References
Sep 21, 1989·Nature·M J Berridge, R F Irvine
Sep 1, 1993·Molecular and Cellular Biology·X LiuT Pawson
Feb 15, 1995·European Journal of Pharmacology·J P WangC M Teng
Feb 1, 1993·Trends in Biochemical Sciences·A W Segal, A Abo
Mar 15, 1996·The Journal of Biological Chemistry·J El BennaB M Babior
Oct 11, 1996·The Journal of Biological Chemistry·A PtasznikG M Bokoch
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·B VanhaesebroeckM D Waterfield
May 19, 1997·Biochemical and Biophysical Research Communications·L T Tsao, J P Wang
Aug 15, 1997·The Biochemical Journal·J DominM D Waterfield
May 18, 1999·The Journal of Biological Chemistry·R A BrownP R Shepherd
Jun 11, 1999·The Journal of Biological Chemistry·T AkasakiH Sumimoto
Oct 3, 1999·The Journal of Biological Chemistry·U MaierB Nürnberg
Feb 11, 2000·Science·Z LiD Wu
Feb 11, 2000·Science·E HirschM P Wymann
Mar 10, 2000·European Journal of Pharmacology·L C Chang, J P Wang
Apr 15, 2000·The Journal of Biological Chemistry·C MurgaJ S Gutkind
Jun 23, 2000·The Journal of Pharmacy and Pharmacology·L C Chang, J P Wang
May 5, 2001·The Journal of Biological Chemistry·B D CuevasG B Mills
May 22, 2001·The Journal of Biological Chemistry·C S HiiA Ferrante
Jun 8, 2001·Annual Review of Biochemistry·B VanhaesebroeckM D Waterfield
Jul 4, 2001·Nature Cell Biology·F KanaiM B Yaffe
Jan 1, 2003·The Journal of Cell Biology·Carsten BrockBernd Nürnberg
Mar 28, 2003·Nature Immunology·Shigeo Koyasu
Apr 5, 2003·Biochimica Et Biophysica Acta·Ling-Chu ChangJih-Pyang Wang
Apr 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Carolyn R HoyalBernard M Babior
May 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qingdan ChenKenneth R McLeish
Jul 23, 2003·Trends in Pharmacological Sciences·Matthias P WymannMuriel Laffargue
Sep 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Neeta G ShenoyLarry L Thomas
Jan 31, 2004·Blood·Kamal D PuriThomas G Diacovo
Mar 23, 2004·Annual Review of Immunology·Jonathan A Deane, David A Fruman
Mar 23, 2004·Biochemical and Biophysical Research Communications·Tohru YamamoriMikinori Kuwabara
Feb 19, 2005·Science·D D SarbassovDavid M Sabatini
Mar 23, 2005·Current Opinion in Cell Biology·James R Woodgett
Apr 5, 2005·British Journal of Pharmacology·Yu-Hsiang KuanJih-Pyang Wang
May 10, 2005·Blood·Alison M CondliffePhillip T Hawkins
Citations
Oct 3, 2013·PloS One·Manuj TandonQiming Jane Wang
Aug 5, 2006·Biochemical Pharmacology·Yu-Hsiang KuanJih-Pyang Wang
Oct 24, 2007·Methods in Enzymology·Amber C DonahueDavid A Fruman
May 14, 2008·Free Radical Research·Cecilia PrataLaura Landi
Feb 3, 2009·Chemical & Pharmaceutical Bulletin·Jin QiBo-Yang Yu
Sep 4, 2019·Organic & Biomolecular Chemistry·Rachel M Gillard, Margaret A Brimble