Activation of phospholipase A2 by endothelin in cultured vascular smooth muscle cells

Biochemical and Biophysical Research Communications
T J ResinkF R Bühler

Abstract

The ability of endothelin to promote phospholipid hydrolysis has been studied in myo-[2-3H]-inositol-, [3H]-arachidonic acid- or methyl-[3H]choline chloride-prelabelled cultured vascular smooth muscle cells (VSMC) from rat and bovine thoracic aortae and human omental vessels. The biochemical responses to endothelin were comparable between the different VSMC isolates. Endothelin promoted the accumulation of glycerolphospho[3H]inositol and concomitant loss of [3H]-inositol label from phosphatidylinositol. Exposure of [3H]choline-labelled VSMC to endothelin resulted in a loss of radioactivity from phosphatidylcholine that was inversely parallelled by an increase in water-soluble [3H]-choline metabolites. In [3H]-arachidonic acid ([3H]-AA)-labelled VSMC, endothelin induced extracellular release of [3H]-AA which derived from both phosphatidylcholine and phosphatidylinositol. Half-maximally effective concentrations of endothelin for all these responses were approximately 2-7 nM and did not vary between VSMC types. Endothelin-induced release of [3H]-AA into VSMC medium-overlay was inhibited by quinacrine and nordihydroguaiaretic acid but not by neomycin or indomethacin. The data herein implicate activation of phospholipase A2 by endot...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P A JonesW Gevers
Jun 1, 1986·Experimental Cell Research·T Scott-BurdenP A Jones
Sep 1, 1985·The Journal of Clinical Investigation·J A Shayman, A R Morrison
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R M BurchJ Axelrod
Aug 15, 1988·Biochemical and Biophysical Research Communications·Y HirataS Sakakibara
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Sep 30, 1980·Biochimica Et Biophysica Acta·H van den Bosch
Jul 1, 1984·Cell·P W MajerusD B Wilson
Apr 15, 1982·The Biochemical Journal·R F Irvine
Jan 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·T Scott-BurdenW Gevers

❮ Previous
Next ❯

Citations

Jan 1, 1997·Medicinal Research Reviews·M L Webb, T D Meek
Mar 1, 1994·Journal of Cellular Physiology·M S GoligorskyW F Bahou
Jul 1, 1992·Medicinal Research Reviews·T MasakiK Goto
Mar 1, 1991·Pflügers Archiv : European journal of physiology·U Klöckner, G Isenberg
Apr 1, 1990·Neurochemical Research·W Lovenberg, R C Miller
Nov 14, 1989·European Journal of Pharmacology·R G FerrarioC Patrono
Dec 1, 1990·Prostaglandins·M D MitchellS S Edwin
Jan 1, 1995·Pharmacology & Therapeutics·M Sokolovsky
Oct 1, 1991·Regulatory Peptides·M A Brown, P L Smith
Jun 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·I T CameronA P Davenport
Jul 5, 2001·European Journal of Pharmacology·A J TrangM E Ward
Nov 21, 1998·Journal of Hepatology·A GabrielC R Gandhi
Sep 1, 1993·Baillière's Clinical Haematology·P E Chabrier
Feb 15, 2001·Progress in Retinal and Eye Research·I O HaefligerT F Lüscher
Jun 19, 2002·Biochimica Et Biophysica Acta·Daniela CordaChristopher P Berrie
Jan 1, 1992·Essays in Biochemistry·T Masaki, M Yanagisawa
May 1, 1995·Clinical and Experimental Pharmacology & Physiology·M La, J J Reid
Jan 1, 1992·The European Journal of Neuroscience·Martine TencéJoël Prémont
Mar 1, 1993·British Journal of Pharmacology·M E RogersonG H Neild
Apr 1, 1990·Acta Physiologica Scandinavica·K P OhmanH R Keiser
May 1, 1991·American Journal of Respiratory Cell and Molecular Biology·J TamaokiT Takizawa
Feb 1, 1992·American Journal of Respiratory Cell and Molecular Biology·T WuJ H Shelhamer
Jan 1, 1992·Journal of Neurosurgery·I YamauraH Shindo
Jul 1, 1990·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·V LagenteP Braquet
May 1, 1997·European Journal of Pharmacology·Y MasudaT Ohtaki
Jul 1, 1991·American Journal of Obstetrics and Gynecology·M D MitchellS S Edwin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.