PMID: 11929305Apr 4, 2002Paper

Activation of polyphenol oxidase in extracts of bran from several wheat (Triticum aestivum) cultivars using organic solvents, detergents, and chaotropes

Journal of Agricultural and Food Chemistry
Moses Okot-KotberKatherine Bagorogoza

Abstract

Polyphenol oxidase (PPO), known to induce browning in wheat-based products, has been shown to be activatable in wheat (Triticum aestivum) bran extracts by chemical compounds. The activity in the extracts could be increased to varying degrees with acetone, methanol, ethanol, 2-propanol, and n-butanol as additives in the extraction buffer. The most potent alcoholic activator was n-butanol (about a 3-fold increase), followed by 2-propanol and ethanol, whereas methanol had the least effect. Ionic detergents in the extraction buffer were also good activators, with sodium dodecyl sulfate (SDS) being more potent (3-fold increase) than cetyltrimethylammonium bromide (CTAB) that had only half as much effect, whereas the nonionic detergent, Triton X-114, was ineffective. The chaotropes, urea and guanidine x HCl (GND), were the most potent activators of all, increasing the activity over 4-fold. Of the two chaotropes, GND was more effective at lower concentrations (<6 M) than urea. However, the enzyme activity lessened at a higher concentration of GND (6 M), while there was a further increase in the activity with 6 M urea treatment. The activity lessened with higher concentration of GND presumably as a result of extensive denaturation of t...Continue Reading

References

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Citations

Apr 26, 2012·Applied Biochemistry and Biotechnology·Nisaporn MuhamadNunta Churngchow
Jun 24, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·Halis SakiroğluMustafa Erat
Jul 19, 2012·Journal of Enzyme Inhibition and Medicinal Chemistry·Arleta Rifati NixhaOktay Arslan
Apr 18, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Hayriye GençOktay Arslan
Sep 12, 2013·Journal of the Science of Food and Agriculture·Alyssa Hidalgo, Andrea Brandolini
May 3, 2014·Journal of Agricultural and Food Chemistry·Robert E AsenstorferDaryl J Mares
Mar 6, 2009·Veterinary Pathology·G LanteriB Macrì

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