Activation of proinflammatory response in human intestinal epithelial cells following Vibrio cholerae infection through PI3K/Akt pathway

Canadian Journal of Microbiology
Arunava BandyopadhayaKeya Chaudhuri

Abstract

Vibrio cholerae activates proinflammatory response in cultured intestinal epithelial cells. In this study, we demonstrate that V. cholerae O395 infection of intestinal epithelial cells results in the activation of Akt. Inhibition of Akt significantly decreases IL-1alpha, IL-6, and TNF-alpha production in V. cholerae infected Int407 cells. Analysis of the mechanisms of Akt influences on cytokine response demonstrates that Akt promotes NF-kappaB activation. We have extended these findings to show that Akt activation may be regulated by bacterial genes associated with virulence, adherence, or motility. Insertion mutants in the virulence genes coding for CtxA, ToxT, and OmpU of V. cholerae modulate the activation of PI3K/Akt signaling pathway, whereas an aflagellate non-motile mutant (O395FLAN) and a adherent and less motile mutant (O395Y3N/O395Y4N) of V. cholerae both show very significant down-regulation of Akt activity in Int407 cells. Together, these observations indicate that Akt promotes proinflammatory cytokine production by V. cholerae infected human intestinal epithelial cells through its influences on NF-kappaB.

References

Aug 1, 1976·The American Journal of Digestive Diseases·G PastoreO Schiraldi
Nov 1, 1995·Infection and Immunity·V SperandioJ B Kaper
Jan 1, 1995·Clinical Microbiology Reviews·J B KaperM M Levine
May 1, 1993·Infection·Y LermanY L Danon
Jan 1, 1996·Journal of Bacteriology·S R ChakrabartiJ Das
Nov 1, 1995·Infection·H BilgenD Molbay
Mar 1, 1996·Infection·H M HasselhornF W Tiller
Jun 8, 1999·The Journal of Biological Chemistry·K IretonP Cossart
Sep 15, 1999·Nature·J A Romashkova, S S Makarov
Dec 2, 1999·Genes & Development·S R DattaM E Greenberg
Sep 9, 2000·The Journal of Biological Chemistry·O Steele-MortimerB B Finlay
Oct 5, 2001·Nature Reviews. Molecular Cell Biology·M P Scheid, J R Woodgett
Mar 5, 2002·Clinical and Diagnostic Laboratory Immunology·Firdausi QadriAnn-Mari Svennerholm
Jul 20, 2002·Biochemical and Biophysical Research Communications·Soumita DasKeya Chaudhuri
Oct 3, 2002·Nature Reviews. Immunology·Qiutang Li, Inder M Verma
Nov 7, 2002·Cell·Derek P BrazilBrian A Hemmings
Mar 1, 1960·The American Journal of Tropical Medicine and Hygiene·E F GANGAROSAP PIYARATN
Dec 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Muriel PichavantPhilippe Gosset
Dec 23, 2003·Infection and Immunity·Xin ZhouJames B Kaper
Mar 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Susan M Butler, Andrew Camilli
May 27, 2005·Organic & Biomolecular Chemistry·Peter Wipf, Robert J Halter
Oct 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Murugesan V S RajaramSusheela Tridandapani
Feb 20, 2007·Infection and Immunity·Carl-Fredrik FlachJan Holmgren
Jun 26, 2007·The International Journal of Biochemistry & Cell Biology·Arunava BandyopadhayaKeya Chaudhuri
Jul 31, 2007·Future Microbiology·Brandon M Childers, Karl E Klose
Sep 11, 2007·Biological & Pharmaceutical Bulletin·Kaoru HazekiOsamu Hazeki

❮ Previous
Next ❯

Citations

Jun 27, 2013·Infection and Immunity·Jessica Queen, Karla J F Satchell
Jun 24, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thomas J CremerSusheela Tridandapani
Oct 27, 2020·Frontiers in Cellular and Infection Microbiology·Thandavarayan RamamurthyAmit Ghosh

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.