Activation of protein kinase C as a modulator of potentiated UK-14304-induced contractions in dog mesenteric artery and vein

Journal of Cardiovascular Pharmacology
H ShimamotoE E Daniel

Abstract

We assessed the role of protein kinase C (PKC) in the mechanism responsible for the potentiation of UK-14304-induced contractions produced when isolated dog mesenteric vascular rings were pretreated with threshold concentrations of 12-O-tetradecanoyl-phorbol-13-acetate (TPA), KCl, or endothelin-1 (ET-1). In dog mesenteric artery. UK-14304 produced a biphasic concentration-response curve in the presence of TPA, KCl, or ET-1, with the curve portion at lower concentrations being alpha 2-adrenoceptor dependent and the portion at higher concentrations being alpha 1-adrenoceptor dependent. Calphostin C (10(-6)M), a PKC inhibitor, abolished amplified UK-14304-induced contraction in the TPA-pretreated tissues. In the KCl- and ET-1-pretreated tissues. 10(-6)M calphostin C antagonized amplified UK-14304-induced contractions by approximately 20% in both parts of the concentration-response curve. In contrast, in dog mesenteric vein, amplified UK-14304-induced contractions by TPA, KCl, and ET-1 were entirely dependent on alpha 2-adrenoceptors. Calphostin C (10(-6)M), which in control experiments had no effect on KCl-induced contraction and antagonized responses to TPA by 60.1%, inhibited UK-14304-induced contraction by 18.3%. Amplified UK-1...Continue Reading

Citations

Apr 15, 2014·Journal of Vascular Surgery·Nathan R TykockiStephanie W Watts
Jun 12, 2001·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·H Suenaga, K Kamata
Oct 19, 2017·Scientific Reports·Ingmar LautenschlägerStefan Uhlig

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