Activation of protein kinase C by coexisting diacylglycerol-enriched and diacylglycerol-poor lipid domains

Biochemistry
A K HinderliterJ J Sando

Abstract

To test the hypothesis that activation of protein kinase C (PKC) is related to the interface between coexisting diacylglycerol- (DAG-) enriched and DAG-poor phases, the thermotropic phase behavior of the ternary mixtures dimyristoylphosphatidylcholine (DMPC)/dimyristoylphosphatidylserine (DMPS)/dioleoylglycerol (DO), DMPC/DMPS/1-palmitoyl-2-oleoylglycerol (PO), and DMPC/DMPS/dimyristoylglycerol (DM) was analyzed and compared with the ability of the lipid mixtures to support PKC activity. Differential scanning calorimetry (DSC) was used to monitor the gel-to-liquid crystalline phase transition as a function of the mole fraction of DO (chiDO), PO (chiPO), or DM (chiDM) in DMPC/DMPS (1:1) multilamellar vesicles (MLVs) and of chiDO in large unilamellar vesicles (LUVs). The addition of DAG at low mole fractions gave rise to the appearance of two or more overlapping transitions. The phase boundaries of the ternary mixtures deduced from the partial phase diagrams were chiDO = approximately 0.10 and approximately 0.3 for DMPC/DMPS/DO, chiPO = approximately 0.05 and approximately 0.4 for DMPC/DMPS/PO, and chiDM = approximately 0.025 and approximately 0.5-0.6 for DMPC/ DMPS/DM. Above these mole fractions of DAG, the transitions again bec...Continue Reading

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Citations

Jun 6, 2002·Chemistry and Physics of Lipids·Rumiana Koynova, Martin Caffrey
Jul 9, 1999·Progress in Lipid Research·F M Goñi, A Alonso
Sep 28, 1998·Biochimica Et Biophysica Acta·A C Newton, J E Johnson
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Mar 7, 2009·Chemical Reviews·Stefan Balaz
Jun 15, 2007·Chemistry and Physics of Lipids·Juan C Gómez-Fernández, Senena Corbalán-García
Jul 1, 2006·Biochimica Et Biophysica Acta·Senena Corbalán-García, Juan C Gómez-Fernández
Jan 27, 1999·Biochimica Et Biophysica Acta·A K HinderliterC E Creutz
Oct 2, 2007·Biophysical Journal·Carla M Rosetti, Bruno Maggio
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Oct 28, 2006·Biochimica Et Biophysica Acta·Pablo V EscribáOliver Vögler
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Mar 15, 2005·Molecular Membrane Biology·Juan C Gómez-FernándezSenena Corbalán-García

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