Activation of protein kinase C contributes to the isoflurane-induced improvement of functional and metabolic recovery in isolated ischemic rat hearts.

Anesthesia and Analgesia
Pengcheng XuIra S Kass

Abstract

Isoflurane enhances myocardial functional recovery and improves energy levels after ischemia. We sought to determine whether isoflurane-induced cardioprotection is mediated by protein kinase C (PKC). The Langendorff model was used, and isolated perfused rat hearts were separated into untreated, isoflurane, chelerythrine (PKC inhibitor) plus isoflurane, and chelerythrine groups. All hearts were subjected to treatment before ischemia, followed by 30 min of ischemia and 60 min of reperfusion. We recorded hemodynamic variables, measured metabolites by high-performance liquid chromatography, and analyzed subcellular localization of PKC isoforms by Western blot analysis. Isoflurane significantly improved the recovery of left ventricular developed pressure, attenuated the depletion of myocardial adenosine triphosphate (ATP) and creatine phosphate at 15 min of ischemia, enhanced the recovery of myocardial ATP and creatine phosphate concentrations after ischemia, and was associated with the translocation of PKC-delta and -epsilon to the membrane. Chelerythrine suppressed the translocation of PKC-delta and -epsilon and blocked the improvement of cardiac function and ATP. We conclude that isoflurane delays the decrease in ATP during ische...Continue Reading

References

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Citations

Jun 2, 2011·Cold Spring Harbor Perspectives in Biology·Peter Lipp, Gregor Reither
Jun 4, 2010·American Journal of Physiology. Cell Physiology·Filip SedlicZeljko J Bosnjak
Nov 30, 2013·Acta Physiologica·R F MapangaM Faadiel Essop
Dec 14, 2006·The American Journal of Chinese Medicine·Xiang-Jian ChenJi-Nan Zhang
Jun 11, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Paul R HanlonElizabeth Murphy
Nov 1, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Rudo F Mapanga, M Faadiel Essop

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