PMID: 9175259May 1, 1997Paper

Activation of protein kinase C enhances the infection of endothelial cells by human cytomegalovirus

Virus Research
M E Slobbe-van DrunenC A Bruggeman

Abstract

The infection of cultured endothelial cells with human cytomegalovirus (HCMV) is generally limited to less than 10% of the cells in contrast to HCMV infection of fibroblasts, where essentially all cells can be infected. It is known that HCMV infection influences a number of signal transduction pathways of infected cells. We therefore questioned whether, conversely, the infectivity of human umbilical vein endothelial cells could be influenced by the deliberate activation of these pathways. When endothelial cells were treated prior to infection with phorbol myristoyl acetate, an activator of protein kinase C, the number of HCMV-positive cells increased two to three times. On the other hand, pretreatment of the cells with RO 31-8220, a specific protein kinase C inhibitor, or with staurosporine, a general protein kinase inhibitor, resulted in a decreased infection level and in abolishment of the PMA-induced effect. Pretreatment with the protein phosphatase inhibitor, okadaic acid, caused a slight increase in infectivity, whereas pretreatment with the protein tyrosine kinase inhibitor, genistein, was without effect. Furthermore, neither forskolin and ilomedine, compounds known to activate the endothelial adenylate cyclase, nor the c...Continue Reading

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Citations

Oct 24, 2007·Journal of Biosciences·Zhengrong SunYujing Huang
Nov 7, 1999·Antiviral Research·C A BruggemanG Nelissen-Vrancken
Oct 28, 1999·American Heart Journal·C Bruggeman
Sep 24, 2004·Microbes and Infection·David L EversEng-Shang Huang
Feb 22, 2005·The Journal of General Virology·James R CarlsonPeter A Barry
Jul 22, 2005·The Journal of General Virology·Natalia KucićPero Lucin

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