PMID: 9178828May 16, 1997Paper

Activation of protein kinase C prevents induction of apoptosis by geranylgeraniol in human leukemia HL60 cells

International Journal of Cancer. Journal International Du Cancer
Y MasudaK Nakaya

Abstract

In a previous study, we showed that geranylgeraniol (GGO) is a potent inducer of apoptosis in human leukemia cells, including HL60 promyelocytic leukemia cells. The present study describes the effects of activators of protein kinase C (PKC) on GGO-induced apoptosis in various lines of leukemia cells. Both 12-O-tetradecanoylphorbol-13-acetate (TPA) and diacylglycerol (DG) inhibited the GGO-induced morphological changes that are characteristic of apoptosis and the DNA fragmentation. Similar effects were observed with other lines of human and murine leukemia cells such as ML1, U937, M1 and P388. Flow cytometric analysis also revealed that both TPA and DG prevented GGO-induced DNA degradation in a dose-dependent manner. These inhibitory effects of TPA and DG were antagonized by inhibitors of PKC such as H-7 and staurosporin, and by amiloride, an inhibitor of Na+/H+ antiporter. In contrast to the inhibitory effects of TPA and DG on GGO-induced apoptosis, 4alpha-TPA, which is unable to activate PKC, failed to prevent GGO-induced DNA fragmentation. However, the selective activator of PKC-beta, 12-deoxyphorbol 13-phenylacetate 20-acetate, significantly inhibited GGO-induced DNA fragmentation. Our results suggest that PKC, and in partic...Continue Reading

References

Jan 15, 1979·Analytical Biochemistry·C F BrunkT W James
Jul 31, 1992·Biochemical and Biophysical Research Communications·M A Barry, A Eastman
Apr 3, 1992·Cell·G I EvanD C Hancock
Jan 1, 1992·Radiation and Environmental Biophysics·A A SeilievK P Hanson
Jan 19, 1990·Science·C C FarnsworthJ A Glomset
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Jan 1, 1995·Journal of Biochemistry·H OhizumiK Nakaya
Feb 1, 1994·Trends in Biochemical Sciences·L V Dekker, P J Parker

❮ Previous
Next ❯

Citations

Dec 16, 2011·Journal of Cellular Physiology·Chih-Chiang ChienYen-Chou Chen
Jun 30, 2000·The Journal of Biological Chemistry·M KratzmeierD Doenecke
Jul 6, 2004·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Kazuyasu Nakaya
Jun 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y Lee, E Shacter
Jun 27, 1998·The Journal of Biological Chemistry·C Y ChenD V Faller
May 5, 2011·Experimental Biology and Medicine·Rajasekhar KaturuHuanbiao Mo
Sep 6, 2013·Experimental Biology and Medicine·Nicolle V FernandesHuanbiao Mo
May 29, 1997·Biochemical and Biophysical Research Communications·Y MasudaK Nakaya

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.