Activation of protein kinase C triggers its ubiquitination and degradation.

Molecular and Cellular Biology
Z LuD A Foster

Abstract

Treatment of cells with tumor-promoting phorbol esters results in the activation but then depletion of phorbol ester-responsive protein kinase C (PKC) isoforms. The ubiquitin-proteasome pathway has been implicated in regulating the levels of many cellular proteins, including those involved in cell cycle control. We report here that in 3Y1 rat fibroblasts, proteasome inhibitors prevent the depletion of PKC isoforms alpha, delta, and epsilon in response to the tumor-promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Proteasome inhibitors also blocked the tumor-promoting effects of TPA on 3Y1 cells overexpressing c-Src, which results from the depletion of PKC delta. Consistent with the involvement of the ubiquitin-proteasome pathway in the degradation of PKC isoforms, ubiquitinated PKC alpha, delta, and epsilon were detected within 30 min of TPA treatment. Diacylglycerol, the physiological activator of PKC, also stimulated ubiquitination and degradation of PKC, suggesting that ubiquitination is a physiological response to PKC activation. Compounds that inhibit activation of PKC prevented both TPA- and diacylglycerol-induced PKC depletion and ubiquitination. Moreover, a kinase-dead ATP-binding mutant of PKC alpha c...Continue Reading

References

Jan 1, 1992·Pharmacology & Therapeutics·J DiGiovanni
Jan 1, 1988·Advances in Cancer Research·S H Yuspa, M C Poirier
Jun 15, 1987·The Biochemical Journal·S YoungS Stabel
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·G ScholzH Hanafusa
Feb 28, 1994·Biochemical and Biophysical Research Communications·M GschwendtF Marks
Aug 30, 1996·The Journal of Biological Chemistry·H W LeeJ B Smith
Jan 1, 1997·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·M RolfeM Pagano
May 16, 1997·The Journal of Biological Chemistry·Q ZangD A Foster
Jun 1, 1997·Molecular and Cellular Biology·Z LuD A Foster

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Citations

Jan 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Haitao JiZhimin Lu
Nov 14, 2015·Molecular Biology of the Cell·Yan WangYong Li
Jul 12, 2013·Journal of Applied Toxicology : JAT·Raymond PoonGuillaume Pelletier
Sep 11, 2004·Biochemical and Biophysical Research Communications·David DurrantRay M Lee
Oct 31, 2006·Biochemical and Biophysical Research Communications·Munehiro NakamuraKazuhiro Iwai
Mar 7, 2006·Mutation Research·Caroline HealyCraig Parfett
Feb 19, 2008·Biochemical and Biophysical Research Communications·Mong-Lien WangIvan Gout
Apr 29, 2014·Circulation Journal : Official Journal of the Japanese Circulation Society·Zhe HaoJun Ge
Jun 20, 2015·Journal of Immunology Research·Rouba Hage-SleimanGhassan Dbaibo
Sep 10, 2013·Cell Cycle·Weiwei Yang, Zhimin Lu
Sep 13, 2008·The International Journal of Biochemistry & Cell Biology·J GwalterI Gout
Aug 22, 2001·Chemistry & Biology·A F Kisselev, A L Goldberg
Aug 17, 2010·Biochimica Et Biophysica Acta·Chagit BrandSanford R Sampson
Apr 29, 2006·Diabetes·María D López-AvalosGordon C Weir
May 1, 2016·Cancer Letters·Nurmaa Dashzeveg, Kiyotsugu Yoshida
May 3, 2003·Biochemical and Biophysical Research Communications·Y Schavinsky-KhrapunskyA Torgeman
Jun 6, 2009·Annual Review of Biochemistry·Zhimin Lu, Tony Hunter
Aug 23, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kumiko TorisuYusaku Nakabeppu
Feb 21, 2004·European Journal of Pharmacology·Ken ShimamuraToshihiko Murayama
Apr 18, 2009·Molecular Aspects of Medicine·Tobias JungTilman Grune

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