PMID: 8958127Jan 1, 1997Paper

Activation of protein phosphorylation by oxidants in vascular endothelial cells: identification of tyrosine phosphorylation of caveolin

Free Radical Biology & Medicine
S VepaV Natarajan

Abstract

Oxidants play a significant role in endothelial cell dysfunction through modulation of diverse biochemical reactions and signal transduction pathways. Towards understanding the role of oxidants in vascular injury, we studied the effect of hydrogen peroxide (H2O2), vanadate, and pervanadate (V(4+)-OOH) on [32Pi] uptake and protein phosphorylation in bovine pulmonary artery endothelial cells (BPAEC). The incorporation of labelled [32Pi] into BPAEC was dependent on the concentration of the oxidant employed and time of incubation. Of the oxidants tested, pervanadate (10 microM) induced maximum incorporation of [32Pi] into cells (two- to threefold over control) followed by H2O2 (1 mM) and vanadate (100 microM) and clear differences in labeled protein profiles were noticed between control and oxidant treated cells. The proteins, analyzed by SDS-PAGE, showed distinct increases in labeling patterns ranging from 21-205 kDa, as evidenced by autoradiography. While the majority of the incorporated [32Pi] was in serine/threonine residues, immunoprecipitation and immunoblotting of cell lysates, using an antiphosphotyrosine antibody, revealed that oxidant treatment resulted in significant increases in total protein tyrosine phosphorylation. M...Continue Reading

References

Nov 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·J R Glenney, D Soppet
Oct 30, 1992·Biochemical and Biophysical Research Communications·D Hecht, Y Zick
Feb 21, 1992·Cell·K G RothbergR G Anderson
Jan 1, 1989·Free Radical Biology & Medicine·I A ClarkW B Cowden
Jan 1, 1989·Free Radical Biology & Medicine·N J Schisler, S M Singh
Jun 1, 1986·Chest·K L Brigham
Sep 1, 1986·Journal of Cellular Physiology·K MackieJ S Lazo
Jun 30, 1995·The Journal of Biological Chemistry·S LiM P Lisanti
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·M ChunH F Lodish
Jul 1, 1994·The Journal of Cell Biology·W J ChangR G Anderson
Jun 1, 1994·Free Radical Biology & Medicine·A BarchowskyK P Chepenik
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R G Anderson

❮ Previous
Next ❯

Citations

May 24, 1996·Chemistry and Physics of Lipids·V NatarajanS Vepa
Mar 4, 2000·Free Radical Biology & Medicine·R G Allen, M Tresini
Dec 14, 2002·The International Journal of Biochemistry & Cell Biology·Thomas Koeck, Karl Kremser
Jan 23, 2002·The Biochemical Journal·Marie-Odile ParatPaul L Fox
Mar 7, 2001·Antioxidants & Redox Signaling·N L ParinandiV Natarajan
Aug 7, 2007·Antioxidants & Redox Signaling·Claudia Alexandra DumitruErich Gulbins
Dec 19, 2012·Antioxidants & Redox Signaling·Alberto Bindoli, Maria Pia Rigobello
Apr 10, 1999·Molecular Biology of the Cell·R Nomura, T Fujimoto
Aug 5, 2011·Molecular Biology of the Cell·James R WhitefordJohn R Couchman
Jun 21, 2002·Annals of the New York Academy of Sciences·Fulvio Ursini, Alex Sevanian
Jan 21, 2011·Journal of Virology·Bernardo A Mainou, Terence S Dermody
Oct 6, 1998·Annual Review of Biochemistry·R G Anderson
Nov 12, 2013·Antioxidants & Redox Signaling·Jie Li, Qishen Pang
May 29, 2012·Seminars in Cell & Developmental Biology·Shyamal C BirChristopher G Kevil
Sep 18, 2007·Traffic·Michelle M HillMarie-Odile Parat
Dec 10, 1999·Experimental Cell Research·T AokiT Fujimoto
Jun 1, 2001·Nitric Oxide : Biology and Chemistry·T Koeck, K Kremser
Jun 23, 2000·The Journal of Surgical Research·K FujiokaB E Sumpio
Mar 21, 1998·The Journal of Cell Biology·S EsserW Risau
Feb 7, 2009·Blood Cells, Molecules & Diseases·Hanene ZbidiGinés M Salido
Jun 12, 2004·Circulation Research·Jean-Philippe GrattonWilliam C Sessa
Apr 12, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Philippe G FrankMichael P Lisanti
Oct 3, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Tao ChenXian-Jun Yu
Jun 17, 2020·Frontiers in Physiology·Pingnian HeFeng Gao
Nov 14, 1998·The American Journal of Physiology·P W Shaul, R G Anderson
Jul 4, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·V NatarajanN L Parinandi
Nov 8, 2001·American Journal of Physiology. Cell Physiology·C G KevilJ S Alexander
Aug 15, 2001·American Journal of Physiology. Cell Physiology·J L KneplerC M Hart
Dec 18, 1998·The Journal of Biological Chemistry·V L Ott, A C Rapraeger
Sep 5, 1997·The Journal of Biological Chemistry·K SkoreyQ Wang
Apr 13, 1999·The American Journal of Physiology·L H YehB R Alevriadou
Dec 19, 2001·Archives of Biochemistry and Biophysics·N L ParinandiV Natarajan
Sep 22, 2001·Archives of Biochemistry and Biophysics·R G MidwinterC C Winterbourn
Jun 2, 2021·Cell Biochemistry and Biophysics·Viswanathan Natarajan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.