PMID: 11903964Mar 21, 2002Paper

Activation of pyrophosphate:fructose-6-phosphate 1-phosphotransferase by fructose 2,6-bisphosphate stimulates conversion of hexose phosphates to triose phosphates but does not influence accumulation of carbohydrates in phosphate-deficient tobacco cells

Physiologia Plantarum
Alisdair R FernieNicholas J Kruger

Abstract

The aim of this work was to investigate the contribution of fructose 2,6-bisphosphate to the regulation of carbohydrate metabolism under phosphate stress. The study exploited heterotrophic tobacco callus lines expressing a modified mammalian 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase that increased the fructose 2,6-bisphosphate content of the tissue. The phosphate status of two transgenic and one untransformed cell line was perturbed by incubation with 2-deoxyglucose, a phosphate-sequestering agent, and by growth of callus on phosphate-depleted media. 31P-NMR spectroscopy confirmed that both treatments decreased cellular levels of inorganic phosphate and phosphorylated metabolites. Despite large decreases in the amounts of phosphate esters, UDPglucose and adenylates in response to phosphate deficiency, the fructose 2,6-bisphosphate content of each line was unaffected by 2-deoxyglucose and increased during growth on phosphate-limited media. Short-term treatment of callus with 2-deoxyglucose had only minor effects on the carbohydrate status of each line, whereas long-term phosphate deficiency caused an increase in starch and a decrease in soluble sugar content in both transgenic and control lines. There were no consiste...Continue Reading

References

Jul 1, 1995·Plant Physiology·P Wojtaszek, G P Bolwell
Nov 1, 1994·Biochemical Society Transactions·N J Kruger, P Scott
Mar 1, 1994·The Plant Journal : for Cell and Molecular Biology·K GraeveR Scheibe
Oct 3, 1999·The Plant Journal : for Cell and Molecular Biology·M Runquist, N J Kruger

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Citations

Aug 18, 2006·Plant Molecular Biology·Eva M FarréLothar Willmitzer
Nov 20, 2020·Journal of Experimental Botany·D M S B DissanayakaWilliam C Plaxton
Jan 4, 2021·Plant Physiology and Biochemistry : PPB·Ziwei LiTengbo Huang
Sep 27, 2003·Plant Physiology·Ute Roessner-TunaliAlisdair R Fernie
May 13, 2011·Plant Physiology·William C Plaxton, Hue T Tran
Dec 1, 2006·Analytical Chemistry·Danielle Ryan, Kevin Robards

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