PMID: 9556219Apr 29, 1998Paper

Activation of red blood cell glutathione peroxidase and morphological transformation of erythrocytes under the action of tert-butyl hydroperoxide

Biochemistry and Molecular Biology International
L B ZavodnikM Bryszewska

Abstract

Susceptibility of control and diabetic erythrocytes to oxidative stress was measured after incubation with various concentration of tert-butyl hydroperoxide (t-BHP). TBA-reactive substances (TBARS) formed were determined by the method of Stocks & Dormady modified by Jain. GSH and total glutathione were estimated by the procedure of Ellman and Akerboom and Sies. Activity of GSH peroxidase was determined by the method of Martinez et al. Protein SH groups were determined after membrane isolation by the method of Dodge et al. Cell morphology was viewed under phase contrast microscope with a magnification of 500x. All results were analyzed by the unpaired two tailed Student's t-test. Oxidative treatment of erythrocytes with tert-butyl hydroperoxide significantly increases the reaction rate but decreases the affinity for substrate (tert-butyl hydroperoxide). The susceptibility of the enzyme from diabetic erythrocytes to oxidation is higher in comparison with normal cells. The oxidation of cellular reduced glutathione (GSH) is not correlated with oxidation of membranous protein SH-groups. Oxidative damage of erythrocytes induces significant cell morphological transformations.

Citations

Feb 1, 2002·Biochemical and Biophysical Research Communications·Elisabetta StrafaceWalter Malorni
Apr 28, 2009·Chemico-biological Interactions·Irene L LisovskayaFazoil I Ataullakhanov
Oct 31, 2008·Journal of Biochemical and Molecular Toxicology·Mohan SinghRavi Kiran
May 14, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K PrasanthiP S Rajini
Jun 2, 2005·Mutation Research·Elena A LapshinaMaria Bryszewska
Nov 14, 2012·Expert Review of Hematology·Prajwal GyawaliEzekiel Uba Nwose
Feb 11, 2021·Antioxidants·Olga V KosmachevskayaAlexey F Topunov
Jul 28, 2020·Mechanisms of Ageing and Development·Sophia WedelMaria Cavinato

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