Activation of replication origins in phi29-related phages requires the recognition of initiation proteins to specific nucleoprotein complexes.

The Journal of Biological Chemistry
R FreireJ M Hermoso

Abstract

Protein p6 of Bacillus subtilis phage phi29 activates the initiation of viral DNA replication by forming a multimeric nucleoprotein complex at the origins of replication, located at both ends of the linear genome. This activation requires a precise positioning of the protein p6 array with respect to the initiation site. To investigate this activation mechanism, we have purified the phi29 protein p6 counterparts from the related phages Nf and GA-1 and analyzed the formation of complexes with DNA. In the homologous protein p6-DNA complexes the phi29 and Nf protein arrays showed an identical positioning, different than that of the GA-1 protein array. In contrast, in the heterologous complexes the protein showed a different arrangement except in the case of the Nf protein-phi29 DNA complex. We have also purified the proteins involved in the initiation of replication (terminal protein and DNA polymerase) from phages Nf and GA-1 and measured the ability of the different p6 proteins to activate homologous and heterologous replication origins. The results obtained indicate that the activation requires not only the formation of a specific nucleoprotein complex but also its specific recognition by the proteins involved in the initiation ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 1, 1976·European Journal of Biochemistry·J L CarrascosaM Salas
Jan 1, 1991·Methods in Enzymology·W J DixonT D Tullius
Jan 1, 1991·Annual Review of Biochemistry·M Salas
May 25, 1990·Science·M SerranoJ M Hermoso
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M DodsonR McMacken
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·I PrietoJ M Hermoso
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A InnisM A Brow
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Hirokawa
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M A Peñalva, M Salas

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Citations

Apr 25, 2000·Nucleic Acids Research·I GascónM Salas
Sep 18, 2007·Journal of Bacteriology·Martín AlcorloJosé M Hermoso
Nov 8, 2001·Journal of Bacteriology·J A HorcajadasM Salas
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·W J MeijerM Salas
Apr 6, 2006·FEMS Microbiology Reviews·Christoph Weigel, Harald Seitz
Jan 18, 2005·FEMS Microbiology Reviews·Alicia BravoMargarita Salas
Feb 28, 2015·Nucleic Acids Research·Isabel HolgueraMargarita Salas
Aug 23, 2016·Frontiers in Molecular Biosciences·Margarita SalasMiguel de Vega

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