Activation of resin with controllable ligand density via catalytic oxa-Michael addition and application in antibody purification

Journal of Chromatography. a
Fang ChengJingping Qu

Abstract

This article reported a new strategy for resin activation with divinyl sulfone using catalytic oxa-Michael addition in a controllable manner. By screening a variety of organocatalysts, PPh3 and DMAP stand out with high catalytic efficiency in aprotic solutions. X-ray photoelectron spectroscopy (XPS) analysis indicates high reaction efficiency and less side reactions than traditional aqueous reactions, resulting in high activation density. A maximum activation density of 157.5 ± 1.2 μmol/g resin was achieved in 12 h using PPh3 as catalyst, which is 1.5 times higher than the traditional aqueous reactions. Followed by conjugation with a chromatographic ligand, i.e., 4-mercaptoethyl pyridine (MEP), the resin is capable of antibody purification. Using IgG and BSA as model proteins, adsorption isotherms and dynamic binding behavior of the resin samples were investigated. A higher affinity and dynamic binding capacity of IgG was observed on resins with higher ligand density. Finally, the resin samples were applied to the purification of a therapeutic monoclonal antibody from cell culture supernatant. The recovery of the resin samples with high ligand density are 70% higher than those of the commercial resin (MEP HyperCel). Moreover, o...Continue Reading

Citations

Jul 20, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jun RenLingyun Jia
Sep 7, 2019·ACS Applied Materials & Interfaces·Xi-Ming WangJian-Hua Wang
Nov 23, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Mingyang LiQing Wang

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