Activation of retinoic acid receptor signaling coordinates lineage commitment of spontaneously differentiating mouse embryonic stem cells in embryoid bodies

FEBS Letters
Zoltan SimandiLaszlo Nagy

Abstract

Retinoid signaling has been implicated in embryonic stem cell differentiation. Here we present a systematic analysis of gene expression changes in mouse embryonic stem cells (mESCs), during their spontaneous differentiation into embryoid bodies and the effect of all-trans retinoic acid (ATRA) on this process. We show that retinoic acid is present in the serum and is sufficient to activate retinoid signaling at a basal level in undifferentiated mESCs. This signal disappears during embryoid body formation. However exogenously added ATRA resets the spontaneous differentiation programs in embryoid bodies and initiates a distinct genetic program. These data suggest that retinoid signaling not only promotes a particular pathway but also acts as a context dependent general coordinator of the differentiation states in embryonic stem cells.

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Citations

Nov 5, 2013·Seminars in Cell & Developmental Biology·Zoltan SimandiLaszlo Nagy
Jun 5, 2012·The Journal of Biological Chemistry·Emmanuel MoutierIrwin Davidson
May 27, 2014·Stem Cell Research·Ixchelt Cuaranta-MonroyLaszlo Nagy
Oct 9, 2015·Clinical & Experimental Metastasis·Mauricio Rodriguez-Torres, Alison L Allan
Jun 1, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Radosław JanuchowskiMaciej Zabel
Aug 4, 2015·Nature Genetics·Folefac AminkengUNKNOWN Canadian Pharmacogenomics Network for Drug Safety Consortium
Sep 5, 2014·Tissue Engineering. Part C, Methods·Sébastien SartYan Li
May 25, 2011·Biochemical and Biophysical Research Communications·Jeong Soon LeeJung Yul Lim
Aug 9, 2016·Molecular Cell·Zoltan SimandiLaszlo Nagy
Jun 27, 2019·Journal of Cellular and Molecular Medicine·Elvira Immacolata ParrottaGiovanni Cuda
Oct 14, 2021·Tissue Engineering. Part B, Reviews·Yanjun GuanJiang Peng

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