Activation of rhodopsin kinase

The Biochemical Journal
N E McCarthy, Muhammad Akhtar

Abstract

The present study confirms our original assertion that peptides corresponding to the C-terminal sequence of rhodopsin are phosphorylated by rhodopsin kinase (RK), but only in the presence of photo-activated rhodopsin [Rho*, which is functionally equivalent to metarhodopsin II (Meta II)]. Under optimized conditions, the extent of peptide phosphorylation reached up to 60% that of Rho*. Rho* phosphorylation began to plateau within 15 min of the initiation of photolysis, whereas the peptide phosphorylation continued linearly for >60 min. This lack of co-ordination in the phosphorylation of the physiological (Rho*) and synthetic (peptide) substrates necessitated the present study, which showed that RK is activated for peptide phosphorylation not only by Meta II but also by Meta III, as well as by the phosphorylated derivatives of these species, but not by opsin. These results led to the conclusion that all the derivatives of opsin, which contain the Schiff base linkage with the all-trans-retinylidene moiety, retain the ability to activate RK.

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Citations

Nov 28, 2002·International Journal of Cancer. Journal International Du Cancer·Sabine VioletteThécla Lesuffleur
May 14, 2003·Progress in Retinal and Eye Research·Tadao MaedaKrzysztof Palczewski
Apr 3, 2009·Biochemistry·Rachel Sterne-MarrJohn J G Tesmer
Jan 15, 2013·Journal of Chemical Information and Modeling·Mulpuri NagarajuDonald Hamelberg
Aug 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Steven J TuckerDavid J MacEwan
Jan 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Gianluca CestraPietro De Camilli
May 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Leonard J FosterMatthias Mann
Jun 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christian SieboldUlrich Baumann
Apr 15, 2009·The Journal of Biological Chemistry·Chih-Chin HuangJohn J G Tesmer
Jul 10, 2010·Journal of Experimental Botany·Pablo PulidoFrancisco Javier Cejudo
Apr 18, 2003·Biology of Reproduction·Sergiy G ShlykovBarbara M Sanborn
Aug 24, 2010·The EMBO Journal·Cassandra A BoguthJohn J G Tesmer
Jun 11, 2021·Frontiers in Cell and Developmental Biology·Edda S F MattheesJulia Drube
Jun 9, 2005·Experimental Eye Research·Young Hee KimGyeong Jae Cho

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