PMID: 41504Jan 1, 1979

Activation of rotavirus RNA polymerase by calcium chelation

Archives of Virology
J CohenR Scherrer

Abstract

Two types of particles were isolated during purification of rotavirus. Dense (D) particles have a density of 1.38 in CsCl and exhibit spontaneously a fully active endogenous transcriptase. Light (L) particles (density of 1.36 in CsCl) need to be treated with chelating agents to show a polymerase activity. The activation process of L particles was studied under strictly controlled monovalent, divalent, and hydrogen ion concentrations. These experiments demonstrate that i) activation is not affected by the ionic strength ii) activation occurs only at a pH higher than 7.1 iii) a low concentration of chelating agent (40 muM EDTA) is sufficient to activate the enzyme. Treatment of particles with EGTA, which chelates selectively Ca2+, leads to unmasking even in the presence of magnesium, indicating that the concentration of free calcium ions plays a major role in the activation process. Various glycosidases, detergents, and chelating agents were tested in respect to unmasking properties. Of these compound only chelating agents turned out to be efficient. Following activation, two glycopeptides were solubilized. These glycopeptides have an apparent molecular weight of 34,000 and 31,000 daltons and react with concanavalin A. The role o...Continue Reading

References

Apr 1, 1977·Virology·A C Durham, D A Hendry
Apr 1, 1977·Virology·A C Durham, M A Haidar
Apr 1, 1977·Virology·A C DurhamM B Von Wechmar
Sep 1, 1977·The Journal of General Virology·J Cohen
May 1, 1976·The Journal of General Virology·J C Bridger, G N Woode
Oct 1, 1977·The Journal of General Virology·M M Elias
Mar 1, 1978·FEBS Letters·J CohenM Monsigny
Mar 1, 1977·Journal of Virology·D Todd, M S McNulty
Dec 29, 1973·Lancet·T H FlewettH Davies
Jun 1, 1973·The Journal of General Virology·H A Wood
Aug 1, 1974·The Journal of General Virology·K F Chater, D H Morgan
Nov 1, 1971·The Journal of Physiology·P F BakerE B Ridgway
Aug 1, 1970·Journal of Virology·D R Black, C A Knight

Citations

Jan 1, 1986·Archives of Virology·S C RichardsonI H Holmes
Jan 1, 1987·Archives of Virology·H BrüssowV Fryder
Jan 1, 1981·Archives of Virology·J A ShirleyT H Flewett
Jan 1, 1982·Archives of Virology·V Wunderlich, G Sydow
Jan 1, 1986·Archives of Virology·H SuzukiN Ishida
Jan 20, 1988·Journal of Molecular Biology·B V PrasadW Chiu
May 1, 1994·Comparative Immunology, Microbiology and Infectious Diseases·M K IjazK M Elhag
May 1, 1994·Comparative Immunology, Microbiology and Infectious Diseases·M K IjazL A Babiuk
Feb 1, 1995·Comparative Immunology, Microbiology and Infectious Diseases·F SupertiL Seganti
Mar 1, 1995·Antiviral Research·M RíosE Spencer
Oct 1, 1995·Current Opinion in Biotechnology·K L WilliamsM B Slade
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M L Dyall-SmithI H Holmes
Dec 9, 2010·The Journal of Biological Chemistry·Fiona E FlemingBarbara S Coulson
Apr 2, 2005·Viral Immunology·Judith M BallRebecca D Parr
Aug 21, 2009·Journal of Virology·Joshua D YoderP R Dormitzer
Jul 20, 2001·Journal of Virology·P R DormitzerStephen C Harrison
Dec 16, 2010·The EMBO Journal·Ethan C SettembreStephen C Harrison
Oct 1, 1992·Seminars in Cell Biology·G Griffiths, P Rottier
Sep 1, 1989·Molecular and Cellular Probes·O NakagomiN Ikegami
Jul 30, 1986·Virology·M S Shahrabadi, P W Lee
Mar 1, 1987·Virology·K F Ready, M I Sabara
May 1, 1987·Virology·M S ShahrabadiP W Lee
Dec 20, 2005·Journal of Molecular Biology·Sarah L GreigAlok K Mitra
May 8, 2002·The Journal of Clinical Investigation·Ningguo FengH B Greenberg
Jul 13, 2014·Virus Research·Ulrich Desselberger
Nov 18, 2000·Virology·P R DormitzerStephen C Harrison
May 30, 2002·Virology·Victoria PandoSusana López
Sep 23, 2014·Current Opinion in Virology·Chelsea L Gridley, John T Patton
Jan 28, 2009·Journal of the American Chemical Society·Ping JingShigeru Amemiya
Jan 16, 2007·Advances in Virus Research·John T PattonZenobia F Taraporewala
Jul 28, 2016·PloS One·Sonja BialowasLennart Svensson
Sep 28, 2018·Journal of Virology·Eric N SalgadoStephen C Harrison
Jan 1, 1997·Families in Society : the Journal of Contemporary Human Services·F G Reamer
Jul 1, 1994·Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)·S al-Khattabi, A O Oduntan
Jan 1, 1983·Journal of Cellular Biochemistry·B L EricsonM K Estes
Mar 12, 1999·Journal of Virology·E DenisovaE R Mackow

Related Concepts

Calcium
Bos indicus
Edetic Acid, Sodium Salt
Egtazic Acid Sodium Salt
Enzyme Activation
Ethylene Glycols
Hydrogen-Ion Concentration
Magnesium
Osmolality
Polypeptides

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