Activation of salivary secretion: coupling of cell volume and [Ca2+]i in single cells

Science
J Kevin Foskett, J E Melvin

Abstract

High-resolution differential interference contrast microscopy and digital imaging of the fluorescent calcium indicator dye fura-2 were performed simultaneously in single rat salivary gland acinar cells to examine the effects of muscarinic stimulation on cell volume and cytoplasmic calcium concentration ([Ca2+]i). Agonist stimulation of fluid secretion is initially associated with a rapid tenfold increase in [Ca2+]i as well as a substantial cell shrinkage. Subsequent changes of cell volume in the continued presence of agonist are tightly coupled to dynamic levels of [Ca2+]i, even during [Ca2+]i oscillations. Experiments with Ca2+ chelators and ionophores showed that physiological elevations of [Ca2+]i are necessary and sufficient to cause changes in cell volume. The relation between [Ca2+]i and cell volume suggests that the latter reflects the secretory state of the acinar cell. Agonist-induced changes in [Ca2+]i, by modulating specific ion permeabilities, result in solute movement into or out of the cell. The resultant cell volume changes may be important in modulating salivary secretion.

References

Jul 29, 1977·Pflügers Archiv : European journal of physiology·H R KoelzI Schulz
Sep 1, 1975·The American Journal of Physiology·J A MangosT Barber
Jul 1, 1986·The American Journal of Physiology·P A Knauf, N A Mann
Aug 1, 1986·The American Journal of Physiology·B Nauntofte, J H Poulsen
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R L Hudson, S G Schultz
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J K FoskettR J Turner
Jun 1, 1988·The Journal of Membrane Biology·M Manganel, R J Turner
Jul 1, 1988·Pflügers Archiv : European journal of physiology·I S AmbudkarB J Baum
Dec 1, 1986·Pflügers Archiv : European journal of physiology·J R Martinez, N Cassity
Oct 1, 1988·The American Journal of Physiology·J K Foskett
Dec 16, 1987·Biochemical and Biophysical Research Communications·M K McMillianB R Talamo
Jun 15, 1987·Biochemical and Biophysical Research Communications·J E MelvinR J Turner
Jan 1, 1985·The Japanese Journal of Physiology·N IwatsukiA Nishiyama
Jan 1, 1985·Pflügers Archiv : European journal of physiology·J R Martinez, N Cassity
Apr 1, 1974·The Journal of Surgical Research·J J PetersonS D Wilson
Feb 23, 1984·Nature·O H Petersen, Y Maruyama
Jan 1, 1982·Pflügers Archiv : European journal of physiology·J Poggioli, J W Putney

❮ Previous
Next ❯

Citations

Jan 1, 1997·Cell Motility and the Cytoskeleton·E SuzakiS Terakawa
Aug 3, 1999·Journal of Cellular Physiology·N ChaïbJ P Dehaye
Feb 1, 2000·Journal of Cellular Physiology·R R Torgerson, M A McNiven
Feb 1, 1994·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J B Larcombe-McDouallM C Steward
Apr 1, 1995·Pflügers Archiv : European journal of physiology·P PoronnikD I Cook
Oct 1, 1992·Pflügers Archiv : European journal of physiology·B IllekT E Machen
Jul 1, 1991·The Journal of Membrane Biology·Y SuzukiK Sato
Sep 22, 2006·The Journal of Membrane Biology·Kunyan KuangJorge Fischbarg
Jul 10, 2004·Pflügers Archiv : European journal of physiology·Ken-ichi ManabeYasunobu Okada
Oct 6, 2006·Pflügers Archiv : European journal of physiology·Ann-Kristin Hansen, Hilde Kanli Galtung
Oct 27, 1992·Biochimica Et Biophysica Acta·A V Tepikin, O H Petersen
Dec 12, 1991·Biochimica Et Biophysica Acta·B Sarkadi, J C Parker
Jan 1, 1995·General Pharmacology·J P Dehaye, V Verhasselt
Nov 1, 1995·General Pharmacology·Y MarunakaT Nakahari
Nov 6, 1998·Pharmacology & Therapeutics·A R Wright, S A Rees
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J M RommensJ K Foskett
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H FischerT E Machen
Sep 20, 2012·The Journal of Biological Chemistry·Robert J Lee, J Kevin Foskett
Dec 1, 1995·The Journal of General Physiology·H Zhao, S Muallem
Jan 19, 2006·The Journal of General Physiology·Jill Thompson, Ted Begenisich
Oct 24, 2008·Acta Physiologica·I H LambertS F Pedersen
Apr 29, 1998·Annual Review of Physiology·J K Foskett
Sep 29, 2009·American Journal of Physiology. Cell Physiology·Clint PerryIrina I Grichtchenko
Feb 3, 2012·Physiological Reviews·Min Goo LeeShmuel Muallem
Aug 27, 2010·The Journal of Clinical Investigation·Robert J Lee, J Kevin Foskett
Mar 6, 2003·The Japanese Journal of Physiology·Keita HosoiTakashi Nakahari
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·S WaldeggerF Lang
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lene N NejsumSøren Nielsen

❮ Previous
Next ❯

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