PMID: 9450944Mar 7, 1998Paper

Activation of shaker potassium channels. I. Characterization of voltage-dependent transitions

The Journal of General Physiology
N E Schoppa, F J Sigworth

Abstract

The conformational changes associated with activation gating in Shaker potassium channels are functionally characterized in patch-clamp recordings made from Xenopus laevis oocytes expressing Shaker channels with fast inactivation removed. Estimates of the forward and backward rates for transitions are obtained by fitting exponentials to macroscopic ionic and gating current relaxations at voltage extremes, where we assume that transitions are unidirectional. The assignment of different rates is facilitated by using voltage protocols that incorporate prepulses to preload channels into different distributions of states, yielding test currents that reflect different subsets of transitions. These data yield direct estimates of the rate constants and partial charges associated with three forward and three backward transitions, as well as estimates of the partial charges associated with other transitions. The partial charges correspond to an average charge movement of 0.5 e0 during each transition in the activation process. This value implies that activation gating involves a large number of transitions to account for the total gating charge displacement of 13 e0. The characterization of the gating transitions here forms the basis for...Continue Reading

References

May 1, 1991·Pflügers Archiv : European journal of physiology·W StühmerS H Heinemann
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K McCormackB Rudy
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H R Guy, P Seetharamulu
Jan 1, 1989·European Biophysics Journal : EBJ·F Conti, W Stühmer
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Aug 1, 1983·Biophysical Journal·R Horn, K Lange
May 1, 1994·Biophysical Journal·L J DeFelice
Feb 1, 1994·Quarterly Reviews of Biophysics·F J Sigworth
Feb 1, 1994·Current Biology : CB·M LiL Y Jan
Apr 1, 1994·Biophysical Journal·E StefaniF Bezanilla
Feb 1, 1994·Biophysical Journal·E PerozoD M Papazian
Feb 1, 1994·The Journal of General Physiology·T HoshiR W Aldrich
Feb 1, 1994·The Journal of General Physiology·W N ZagottaR W Aldrich
Feb 1, 1994·The Journal of General Physiology·W N ZagottaR W Aldrich
Nov 1, 1993·Biophysical Journal·L Heginbotham, R MacKinnon
Jan 1, 1993·Biophysical Journal·S C Crouzy, F J Sigworth
Mar 1, 1996·Biophysical Journal·D Johnston
Feb 1, 1996·Neuron·H P LarssonE Y Isacoff

❮ Previous
Next ❯

Citations

Dec 23, 2008·Cellular and Molecular Life Sciences : CMLS·J CuiU S Lee
Sep 24, 2004·The Journal of Membrane Biology·A Kargol, A Hosein-Sooklal
May 30, 2001·Progress in Biophysics and Molecular Biology·D Fedida, J C Hesketh
Aug 2, 2002·Trends in Biochemical Sciences·Jeffrey W Karpen, MariaLuisa Ruiz
Nov 20, 2004·Nature Reviews. Neuroscience·Kenton J Swartz
Dec 23, 2008·Nature Structural & Molecular Biology·Yuval Ben-AbuOfer Yifrach
Dec 28, 2010·Nature Structural & Molecular Biology·Sudha ChakrapaniEduardo Perozo
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Boris Martinac, Owen P Hamill
Jun 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Daniel SchmidtRoderick MacKinnon
Sep 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ruhma SyedaHagan Bayley
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Toshinori Hoshi, Clay M Armstrong
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Daniel WerryDavid Fedida
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·R Horn
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·C M Armstrong, G Cota
Apr 2, 2011·The Journal of Biological Chemistry·Andrés Jara-OsegueraLeón D Islas
Oct 6, 1998·The Journal of General Physiology·J Zheng, F J Sigworth
Dec 2, 1998·The Journal of General Physiology·P C Zei, R W Aldrich
Mar 3, 1999·The Journal of General Physiology·J B Patlak
Jul 10, 1999·The Journal of General Physiology·E RíosN Shirokova
Aug 6, 1999·The Journal of General Physiology·F T HorriganR W Aldrich
Dec 14, 1999·The Journal of General Physiology·L D Islas, F J Sigworth
Feb 2, 2000·The Journal of General Physiology·C GonzalezR Latorre
Feb 7, 2001·The Journal of General Physiology·R OlceseE Stefani
Aug 29, 2001·The Journal of General Physiology·H T KurataD Fedida
May 30, 2002·The Journal of General Physiology·David H HackosKenton J Swartz
Oct 1, 2003·The Journal of General Physiology·Ramon LatorreOsvaldo Alvarez
Oct 15, 2003·The Journal of General Physiology·Manana SukharevaKenton J Swartz
Jan 28, 2004·The Journal of General Physiology·Ulrike Laitko, Catherine E Morris
May 19, 2004·The Journal of General Physiology·Jon T SackWilliam F Gilly
Sep 15, 2004·The Journal of General Physiology·Tetsuya KitaguchiKenton J Swartz
Dec 30, 2004·The Journal of General Physiology·Medha PathakEhud Isacoff
Jun 1, 2006·The Journal of General Physiology·Ulrike LaitkoCatherine E Morris
Nov 2, 2005·The Journal of General Physiology·Kenton J Swartz
Sep 27, 2006·The Journal of General Physiology·Xiang QianKarl L Magleby
Nov 30, 2006·The Journal of General Physiology·Kevin Dougherty, Manuel Covarrubias
Jun 27, 2007·The Journal of General Physiology·Andrew Bruening-WrightH Peter Larsson
Oct 17, 2007·The Journal of General Physiology·Sudha ChakrapaniEduardo Perozo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.