Activation of Shiga-like toxins by mouse and human intestinal mucus correlates with virulence of enterohemorrhagic Escherichia coli O91:H21 isolates in orally infected, streptomycin-treated mice.

Infection and Immunity
Angela R Melton-CelsaA D O'Brien

Abstract

The enterohemorrhagic Escherichia coli (EHEC) O91:H21 isolates B2F1 and H414-36/89 are virulent in an orally infected streptomycin-treated mouse model. Previous studies demonstrated that B2F1 and H414-36/89 grow to high levels in mucus isolated from mouse small intestine and colon and that growth in small-intestine mucus is related to virulence. We measured the levels of Shiga-like toxins (SLTs) SLT-IIvha and SLT-IIvhb produced by B2F1 after growth in Luria-Bertani (LB) broth supplemented with mouse intestinal mucus by assaying the cytotoxicity of culture supernatants on Vero cells. Culture supernatants from B2F1 grown in mouse intestinal mucus, but not EHEC strains that produce SLT-II or SLT-IIc, were approximately 35- to 350-fold more toxic for Vero cells than supernatants from B2F1 grown in LB broth. This increased toxicity was not reflected by a concomitant increase in SLT antigen content. Furthermore, when culture supernatants from B2F1 or K-12 strains carrying plasmids encoding SLTs cloned from H414-36/89 or purified SLT-IIvhb from B2F1 were incubated with mouse intestinal mucus, the samples exhibited greater cytotoxicity than when they were incubated with N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) buffer...Continue Reading

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Citations

Dec 22, 2007·Journal of Food Protection·Aida Juliana MartínezMaria Consuelo Vanegas
Aug 30, 2008·Applied and Environmental Microbiology·Evelyn A Dean-NystromJoachim F Pohlenz
Mar 13, 2010·Journal of Medical Microbiology·Luis Fernando dos SantosBeatriz Ernestina Cabilio Guth
Jul 1, 2010·Toxins·Oludare OdumosuWilliam Langridge
Jul 9, 2017·Applied and Environmental Microbiology·Peter C H FengLothar Beutin
Apr 30, 1999·Emerging Infectious Diseases·C K SchmittA D O'Brien
Feb 1, 2000·The Journal of Biological Chemistry·J F Kokai-KunA D O'Brien
Jan 17, 2004·Clinical Microbiology Reviews·Herbert Schmidt, Michael Hensel
Jan 29, 2011·Journal of Biomedicine & Biotechnology·Krystle L Mohawk, Alison D O'Brien
Jan 5, 2011·Infection and Immunity·Cynthia A FullerAlison A Weiss
Nov 12, 2015·Journal of Food Protection·Angela R Melton-CelsaPeter C H Feng
Jan 11, 2005·Applied and Environmental Microbiology·Wenlan ZhangHelge Karch
Oct 10, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Martina BielaszewskaHelge Karch
Feb 7, 2008·Expert Review of Anti-infective Therapy·Dorothea OrthReinhard Würzner
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Aug 16, 2008·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Johanna ScheiringLothar Bernd Zimmerhackl

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