PMID: 9421504Feb 28, 1998Paper

Activation of stress-activated MAP protein kinases up-regulates expression of transgenes driven by the cytomegalovirus immediate/early promoter

Nucleic Acids Research
W BrueningH D Durham

Abstract

The immediate/early promoter/enhancer of cytomegalovirus (CMV promoter) is one of the most commonly used promoters for expression of transgenes in eukaryotic cells. In practice, the CMV promoter is often thought of as a constitutively active unregulated promoter. However, we have observed that transcription from the CMV promoter can be up-regulated by a variety of environmental stresses. Many forms of cellular stress stimulate MAP kinase signalling pathways, resulting in activation of stress-activated protein kinases [SAPKs, also called Jun N-terminal kinases (JNKs)] and p38 kinases. We have found that the same conditions that lead to activation of SAPK/JNKs and p38 kinases can also dramatically increase expression from the CMV promoter. Inhibitors of p38 kinases abolished basal transcription from the CMV promoter and completely blocked stress-induced up-regulation of the CMV promoter. Overexpression of a dominant negative JNK kinase had no effect on basal transcription, but significantly reduced up-regulation caused by stress. These results have grave implications for use of the CMV promoter. If the CMV promoter can be up-regulated by cellular stresses, inadvertent activation of the stress kinase pathways may complicate, if no...Continue Reading

References

Jan 1, 1990·The Journal of General Virology·T StammingerB Fleckenstein
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·A S ZervosR Brent
Jul 1, 1995·The American Journal of Medicine·G R SimonsS Z Goldhaber
May 1, 1996·Journal of Virology·J F BaskarP Ghazal
Oct 4, 1996·The Journal of Biological Chemistry·J M Kyriakis, J Avruch
Nov 29, 1996·The Journal of Biological Chemistry·B I Giasson, W E Mushynski

❮ Previous
Next ❯

Citations

Apr 17, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Urska KamensekMaja Cemazar
Aug 19, 2010·Cell Stress & Chaperones·Frank BoschelliKim T Arndt
Oct 26, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M D WheelerR G Thurman
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·M ZeinerU Gehring
Sep 12, 2008·Molecular Biology of the Cell·Yeung-Tung SiuDong-Yan Jin
May 12, 2000·Critical Care Medicine·T ObataM B Yaffe
Jan 30, 2009·Pigment Cell & Melanoma Research·Chi-Ping DayGlenn Merlino
Jul 15, 2006·Journal of Virology·Claudio R SantosPedro A Lazo
Feb 19, 2010·Virology Journal·Marie-Noëlle TakahashiKatherine E Owen
Jan 11, 2014·Expert Review of Anti-infective Therapy·Cecilia Söderberg-Nauclér
May 11, 2004·The Journal of Cell Biology·J M BeaulieuJ P Julien
Sep 1, 2004·Expert Opinion on Biological Therapy·Lina PrasmickaiteKristian Berg
May 3, 2001·Photochemistry and Photobiology·L PrasmickaiteK Berg
Feb 11, 2012·Trends in Molecular Medicine·Marleen KeyaertsTony Lahoutte
Aug 17, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Hsiu-Jen WangRobert S Aronstam
Apr 1, 2008·The International Journal of Biochemistry & Cell Biology·Prakash RadhakrishnanPi-Wan Cheng
Jun 10, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Soo Jung KimMichael Abecassis
Oct 9, 2003·The Journal of Gene Medicine·Rodolphe VereecqueBruno Quesnel
Mar 7, 2006·The Journal of Gene Medicine·Birgit Ø EngesaeterGunhild M Maelandsmo
Feb 26, 2011·Traffic·Dawn Thompson, Jennifer L Whistler
Jul 11, 2006·Biochimica Et Biophysica Acta·Annika Järviluoma, Päivi M Ojala
Feb 8, 2006·Analytical Biochemistry·Madhu S MaloRichard A Hodin
Sep 11, 2016·Biochemical and Biophysical Research Communications·Kitako TabataHiroshi Tomita

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.