Activation of T cell Raf-1 at mitosis requires the protein-tyrosine kinase Lck.

The Journal of Biological Chemistry
N I PathanM L Harrison

Abstract

The serine/threonine protein kinase Raf-1 is activated in response to a variety of growth factors in fibroblasts and hematopoietic cells. In T cells, Raf-1 is activated in response to stimulation through the T cell antigen receptor, the interleukin-2 receptor, and by stimulation of protein kinase C. We demonstrate here that in T cells, Raf-1 is also activated during mitosis. The mitotic activation of Raf-1 was not observed in the Lck-deficient cell line, J.CaM.1. During mitosis, Raf-1 was found to interact selectively with a mitotic form of Lck that migrated with a reduced electrophoretic mobility on SDS-polyacrylamide gels. We conclude that Raf-1 is activated during mitosis in T cells and that this mitotic activation of Raf-1 is dependent on the presence of Lck.

References

Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L E SamelsonR D Klausner
Nov 10, 1995·The Journal of Biological Chemistry·A D LairdD Shalloway
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·B C TurnerJ C Reed
Mar 1, 1996·Molecular and Cellular Biology·T JelinekM J Weber
Jun 1, 1996·Current Opinion in Immunology·A C Chan, A S Shaw
Nov 1, 1996·The Journal of Biological Chemistry·N I PathanM L Harrison

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Citations

May 5, 2001·Pharmacology & Therapeutics·C R Weinstein-OppenheimerJ A McCubrey
Dec 26, 2001·Cytokine & Growth Factor Reviews·Jonathan M Ellery, Peter J Nicholls
Jan 1, 1997·Annual Review of Cell and Developmental Biology·S M Thomas, J S Brugge
Aug 5, 2005·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·Zhuo ZhangHui Wang
Feb 6, 2007·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Michael L H WongChristopher M Hovens
Nov 4, 2015·Journal of Cellular Biochemistry·Erika IwamotoYuji Nakayama
Oct 22, 2014·The FEBS Journal·Su AnTian-Rui Xu
Jun 23, 2006·European Journal of Immunology·Hirotake TsukamotoYasuharu Nishimura
Feb 6, 1999·The Journal of Biological Chemistry·A D LairdD Shalloway
Sep 3, 1998·The Journal of Biological Chemistry·A ZiogasG Radziwill
Jul 21, 1998·The Journal of Biological Chemistry·Y H LingR Perez-Soler
Feb 16, 2002·The Journal of Biological Chemistry·Kamala P KesavanMarietta L Harrison

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