Activation of T lymphocytes by syngeneic murine intestinal smooth muscle cells

Gastroenterology
C M HogaboamS M Collins

Abstract

Intestinal smooth muscle cells (ISMCs) express major histocompatibility complex II (MCH II) and intercellular adhesion molecule 1 (ICAM-1) after exposure to interferon gamma (IFN-gamma). T lymphocytes invade the intestinal musculature during Crohn's disease or pseudoobstruction. The aim of this study was to determine whether ISMCs activate syngeneic T cells via MHC II and ICAM-1. Cultured murine ISMCs were exposed to IFN-gamma for 72 hours and analyzed for Mac-1 (CD11B CD18) antigen, MHC II, and ICAM-1 expression using enzyme-linked immunosorbent assay and fluorescence-activated cell sorter scan. T lymphocytes from mesenteric lymph nodes of ovalbumin-sensitized mice were examined for their ability to proliferate after coculture with IFN-gamma-pretreated and ovalbumin-pretreated ISMCs using [3H]thymidine incorporation. ISMCs expressed smooth muscle alpha-actin before and after IFN-gamma exposure. No macrophages were identified in these cultures. Exposure to IFN-gamma and ovalbumin for 72 hours induced MHC II and ICAM-1 expression; these treated ISMCs induced T-cell proliferation, whereas untreated ISMCs did not. T-cell proliferation was markedly enhanced by adding interleukin 2 and was blocked by antibodies against MHC II and IC...Continue Reading

Citations

Oct 23, 2008·Scandinavian Journal of Gastroenterology·Catherine ReenaersEdouard Louis
Mar 8, 2016·Gastroenterología y hepatología·Eugeni Domènech, Javier P Gisbert
Aug 23, 2012·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·T Shea-DonohueA Zhao
Feb 1, 2014·Alimentary Pharmacology & Therapeutics·T LobatónP Rutgeerts
Jun 25, 2014·Frontiers in Pharmacology·Susan Hua
Dec 25, 2010·Therapeutic Advances in Gastroenterology·Subrata Ghosh, Remo Panaccione
Jul 20, 2002·Inflammatory Bowel Diseases·Gert van Assche, Paul Rutgeerts
Apr 1, 1997·Inflammatory Bowel Diseases·S M CollinsC Hogaboam
Jan 12, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Bruce A VallanceStephen M Collins
Dec 8, 2015·World Journal of Gastroenterology : WJG·Emilio G QuetglasWalter Reinisch
Feb 19, 1998·Alimentary Pharmacology & Therapeutics·D M McKayM H Perdue
Nov 14, 1997·The American Journal of Physiology·C Fiocchi

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