PMID: 11319165Apr 25, 2001Paper

Activation of the Akt/protein kinase B signaling pathway is associated with granulosa cell survival

Biology of Reproduction
A L JohnsonJ A Swenson

Abstract

Follicles from the hen ovary that have been selected into the preovulatory hierarchy are committed to ovulation and rarely become atretic under normal physiological conditions. In part, this is attributed to the resistance of the granulosa layer to apoptosis. The present studies were conducted to evaluate the role of the phosphatidylinositol (PI) 3-kinase/Akt signaling pathway in hen granulosa cell survival and, by implication, follicle viability. Cloning of the chicken akt2 homologue revealed a high degree of amino acid homology to its mammalian counterparts within the catalytic domain, plus complete conservation of the putative Thr(308) and Ser(474) phosphorylation sites. Treatment of granulosa cells from the three largest preovulatory follicles with insulin-like growth factor (IGF)-I and, to a lesser extent, transforming growth factor (TGF)-alpha induces rapid phosphorylation of Akt, and such phosphorylation is effectively blocked by the PI 3-kinase-inhibitor LY294006. Serum withdrawal from cultured cells for 33-44 h initiates oligonucleosome formation, an indicator of apoptotic cell death, whereas cotreatment with IGF-I prevents this effect. Moreover, treatment of cultured cells for 20 h with LY294006 induces apoptosis. The...Continue Reading

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Q ChengJ R Testa
Dec 1, 1991·Cell Regulation·P F JonesB A Hemmings
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P F JonesB A Hemmings
Nov 24, 1995·Science·Z XiaM E Greenberg
Aug 22, 1995·International Journal of Cancer. Journal International Du Cancer·A BellacosaJ R Testa
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·J Q ChengJ R Testa
Jan 1, 1997·Annual Review of Physiology·A Kaipia, A J Hsueh
Nov 22, 1997·Biology of Reproduction·A L JohnsonJ L Tilly
Apr 8, 1998·Current Opinion in Genetics & Development·D R Alessi, P Cohen
Apr 30, 1998·Current Opinion in Cell Biology·J Downward
Nov 13, 1998·Science·M H CardoneJ C Reed
Nov 26, 1998·The Journal of Biological Chemistry·K Du, M Montminy
Feb 18, 1999·Current Biology : CB·C BelhamJ Avruch
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L C Cantley, B G Neel
Jun 9, 1999·Current Biology : CB·L P KaneA Weiss
Jul 27, 1999·Current Biology : CB·R T Peterson, S L Schreiber
Sep 16, 1999·Biochemical and Biophysical Research Communications·R H OliverJ Yeh
Sep 28, 1999·Endocrinology·A L JohnsonT Jensen
Oct 20, 1999·Domestic Animal Endocrinology·O M OnagbesanE Decuypere
Dec 2, 1999·Genes & Development·S R DattaM E Greenberg
Jul 26, 2000·The Journal of Biological Chemistry·J OkanoH Nakagawa

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Citations

Nov 14, 2007·Journal of Assisted Reproduction and Genetics·Maki GotoFumitaka Kikkawa
Nov 19, 2003·Biochemical and Biophysical Research Communications·R SassonA Amsterdam
Oct 14, 2003·Biochemical Pharmacology·R Sasson, A Amsterdam
Jun 24, 2003·Animal Reproduction Science·A L Johnson
Jun 1, 2006·Nutrition Research Reviews·Sophie TesseraudJoëlle Dupont
Oct 7, 2008·Human Reproduction·I Ben-AmiA Amsterdam
Nov 30, 2006·The Journal of Reproduction and Development·Chunhua MengKazuyoshi Taya
Mar 24, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·A L JohnsonDori C Woods
Apr 2, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Dori C WoodsA L Johnson
Feb 16, 2002·Biochemical and Biophysical Research Communications·Jamie T Bridgham, Alan L Johnson
Feb 20, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Maki GotoFumitaka Kikkawa
Jun 21, 2001·Biochemical and Biophysical Research Communications·J T BridghamA L Johnson
May 28, 2013·Reproductive Toxicology·Manjari MishraMukul Das
Dec 9, 2008·General and Comparative Endocrinology·A L Johnson, Dori C Woods
Nov 26, 2008·Animal Reproduction Science·Okanlawon OnagbesanEddy Decuypere
Nov 11, 2008·General and Comparative Endocrinology·J DupontJ Simon
Nov 7, 2006·Molecular and Cellular Endocrinology·Jennifer D CannonCharles L Chaffin
Jul 1, 2011·Topics in Cognitive Science·Laura R NovickKefyn M Catley
May 28, 2011·Reproduction in Domestic Animals = Zuchthygiene·J LinC Zhang
Nov 14, 2014·The Prostate·Shigang XiongJacek Pinski
Feb 27, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Araceli Diez-FraileKatharina G M A D'Herde
Nov 26, 2005·Domestic Animal Endocrinology·Pascal VaudinSophie Tesseraud
Oct 8, 2005·Fertility and Sterility·Barbara SonntagRobert R Greb
Apr 10, 2007·Biochemical and Biophysical Research Communications·Sophie TesseraudIban Seiliez
Apr 19, 2008·Reproductive Sciences·Shilla Chakrabarty, Manubai Nagamani
Aug 24, 2002·Endocrinology·A L JohnsonJ T Bridgham

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