Activation of the avian erythrocyte Na-K-Cl cotransport protein by cell shrinkage, cAMP, fluoride, and calyculin-A involves phosphorylation at common sites.

The Journal of Biological Chemistry
C Lytle

Abstract

Na-K-Cl cotransport activity in duck erythrocytes increases approximately 10-fold in response to osmotic cell shrinkage, norepinephrine, fluoride, or calyculin-A (an inhibitor of type-1 and -2a phosphatases). To assess whether all four stimuli promote phosphorylation of the cotransport protein and whether this phosphorylation is catalyzed by the same kinase, the cotransporter was isolated from erythrocytes by immunoprecipitation and its pattern of phosphorylation was evaluated. Each stimulus evoked proportionate increases in cotransporter activity and phosphorylation. No two stimuli in combination evoked greater activation and phosphorylation than did the more potent of the two stimuli acting alone. Phosphoamino acid analysis of the cotransport protein indicated that phosphorylation occurs at serine and threonine residues. Phosphopeptide mapping revealed a distinctive pattern of 8 major tryptic phosphopeptides, none of which were significantly phosphorylated in the unstimulated state. Maps of cotransporters activated by the four different stimuli were indistinguishable. Measurements of phosphorylation stoichiometry indicated that each cotransporter acquires approximately 5 phosphates on going from an inactive state in swollen c...Continue Reading

References

Oct 1, 1992·The Journal of Clinical Investigation·J B MatthewsJ L Madara
Dec 1, 1990·The Journal of General Physiology·J C ParkerH J Gitelman
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Jan 1, 1988·The American Journal of Physiology·S M O'GradyM Field
Apr 1, 1988·The American Journal of Physiology·A A AltamiranoJ M Russell
Jan 1, 1980·Annals of the New York Academy of Sciences·J M Russell
Jan 1, 1981·Annals of the New York Academy of Sciences·H C Palfrey, P Greengard
Aug 4, 1980·Biochimica Et Biophysica Acta·P GeckE Heinz
Aug 1, 1995·Current Opinion in Cell Biology·J A Payne, B Forbush
Oct 20, 1995·The Journal of Biological Chemistry·A TanimuraR J Turner
Nov 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J W Mills, L J Mandel
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·J C XuB Forbush
May 1, 1993·The Journal of General Physiology·P B DunhamP J Logue
Dec 11, 1995·The American Journal of Physiology·C LytleB Forbush
Feb 11, 1996·The American Journal of Physiology·C Lytle, B Forbush
Nov 15, 1996·The Journal of Biological Chemistry·L D'AndreaJ L Madara
Oct 1, 1995·Journal of Biomedical Science·S.R. Haynes

❮ Previous
Next ❯

Citations

Jul 31, 2001·International Journal of Cancer. Journal International Du Cancer·L MarklundK Grankvist
Jan 3, 2006·Cellular and Molecular Neurobiology·Julia V BukanovaVladimir G Skrebitsky
Jun 1, 2006·Acta Physiologica·E Delpire, K B Gagnon
Oct 24, 2008·Acta Physiologica·I H LambertS F Pedersen
Sep 30, 2010·Acta Physiologica·E K Hoffmann, S F Pedersen
Dec 31, 2005·Molecular and Cellular Biology·Kenneth B E GagnonEric Delpire
Jul 1, 2006·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·G P Gusev, T I Ivanova
Jun 9, 2000·Annual Review of Physiology·M Haas, B Forbush
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Rebecca LewisRichard Barrett-Jolley
Feb 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Paola de Los HerosGerardo Gamba
Dec 25, 2004·The Journal of Physiology·Peter W Flatman
Aug 5, 1999·The Journal of Physiology·P W Flatman, J Creanor
Jun 8, 1999·The Journal of Physiology·P W Flatman, J Creanor
Jun 10, 2010·The Journal of Physiology·Kenneth B Gagnon, Eric Delpire
May 26, 2010·The Journal of Physiology·Eric Delpire, Thomas M Austin
Dec 19, 2008·Experimental Eye Research·Paul J DonaldsonKevin F Webb
Feb 10, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·E K HoffmannW S Marshall
May 6, 2004·Basic & Clinical Pharmacology & Toxicology·Linda MarklundKjell Grankvist
Jun 9, 2005·Experimental Eye Research·Friedrich P DieckeJorge Fischbarg
Aug 1, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Tatiana I IvanovaGennadii P Gusev
Nov 8, 2002·Biochimica Et Biophysica Acta·Peter W Flatman
Jul 20, 2010·Biochimica Et Biophysica Acta·Kristopher T KahleRichard P Lifton
Jul 6, 2005·Biochimica Et Biophysica Acta·Ioulia MatskevichPeter W Flatman
Mar 17, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Gengru JiangW Charles O'Neill
Nov 25, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Geir Øystein AndersenJan-Bjørn Osnes
Jan 30, 2004·American Journal of Physiology. Cell Physiology·Lilia M MaglovaJohn M Russell
Jul 30, 2002·The Journal of Biological Chemistry·Rachel B Darman, Biff Forbush
Feb 25, 2005·American Journal of Physiology. Cell Physiology·Caterina Di Ciano-OliveiraAndrás Kapus
Feb 18, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·S F PedersenP M Cala
Aug 9, 2003·Journal of Neurophysiology·Hao WangDandan Sun
Jan 11, 2003·Journal of Neurophysiology·Stacey L SchombergDandan Sun

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.