Activation of the Bacillus subtilis global regulator CodY by direct interaction with branched-chain amino acids

Molecular Microbiology
Robert P Shivers, Abraham L Sonenshein

Abstract

CodY, a GTP-activated global transcriptional regulator of early stationary phase genes, is conserved in many Gram-positive bacterial species. Recently, a number of novel targets regulated by CodY have been identified, including three Bacillus subtilis operons involved in branched-chain amino acid (BCAA) biosynthesis (Molle, V., et al., 2003, J Bacteriol 185: 1911-1922). The mechanism of involvement of CodY in regulating the ilvB operon was investigated here using in vivo transcriptional fusions, in vitro gel mobility shift assays and DNase I footprinting assays. CodY was found to mediate regulation of the ilvB operon by GTP and BCAAs and to bind to the ilvB promoter region. BCAAs increased the affinity of CodY for the ilvB promoter and for all other CodY targets tested. This effect of BCAAs in vitro was additive with the effect of GTP on CodY DNA-binding activity.

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Citations

Jun 7, 2005·FEMS Microbiology Reviews·Jan KokOscar P Kuipers
Apr 2, 2005·Current Opinion in Microbiology·Abraham L Sonenshein
Dec 23, 2010·Chemical Reviews·Matthew ThoendelAlexander R Horswill
Nov 6, 2007·Nature Reviews. Microbiology·Abraham L Sonenshein
Oct 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Allison V BanseRichard Losick
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Boris R Belitsky, Abraham L Sonenshein
Sep 24, 2010·The Journal of Biological Chemistry·Marat R SadykovGreg A Somerville
Nov 23, 2011·Antimicrobial Agents and Chemotherapy·Swantje ReissSusanne Engelmann
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Apr 7, 2010·Journal of Bacteriology·Charlotte D MajerczykAbraham L Sonenshein
Aug 23, 2006·Journal of Bacteriology·Alisa W SerioAbraham L Sonenshein
Jun 10, 2008·Journal of Bacteriology·José A LemosRobert A Burne
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Jan 18, 2012·Journal of Molecular Microbiology and Biotechnology·Holly Anne MartinEduardo A Robleto
Feb 10, 2009·Bioscience, Biotechnology, and Biochemistry·Yasutaro Fujita

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