Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption

Science
K G HardwickA W Murray

Abstract

The spindle assembly checkpoint keeps cells with defective spindles from initiating chromosome segregation. The protein kinase Mps1 phosphorylates the yeast protein Mad1p when this checkpoint is activated, and the overexpression of Mps1p induces modification of Mad1p and arrests wild-type yeast cells in mitosis with morphologically normal spindles. Spindle assembly checkpoint mutants overexpressing Mps1p pass through mitosis without delay and can produce viable progeny, which demonstrates that the arrest of wild-type cells results from inappropriate activation of the checkpoint in cells whose spindle is fully functional. Ectopic activation of cell-cycle checkpoints might be used to exploit the differences in checkpoint status between normal and tumor cells and thus improve the selectivity of chemotherapy.

References

Mar 21, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D J Clarke, J F Giménez-Abián
Mar 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G J Gorbsky
Sep 28, 2006·Current Genetics·Olga S FedyaninaEkaterina L Grishchuk
Feb 4, 1998·Trends in Genetics : TIG·K G Hardwick
Oct 22, 2003·Archives of Medical Research·Carla S Lopes, Claudio E Sunkel
Dec 1, 1996·Current Opinion in Cell Biology·A D Rudner, A W Murray
Jan 13, 1998·Current Opinion in Cell Biology·Peter K SorgerA A Hyman
Feb 19, 2000·Current Opinion in Genetics & Development·D J Burke
Apr 27, 1999·Trends in Biochemical Sciences·K Nasmyth
Aug 19, 2000·Molecular Cell·G PereiraE Schiebel
Oct 5, 2011·Nature Cell Biology·A W Murray
Jun 5, 2012·Nature Cell Biology·Yuya YamagishiYoshinori Watanabe
Apr 12, 2007·Nature Reviews. Molecular Cell Biology·Andrea Musacchio, Edward D Salmon
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Andrea Musacchio, K G Hardwick
Aug 3, 1999·European Journal of Biochemistry·K A Farr, O Cohen-Fix
Dec 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Daisuke ItoTomohiro Matsumoto
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·H J ChialM Winey
Oct 5, 2010·The Journal of Biological Chemistry·Tingting SunXuedong Liu
Dec 11, 2002·The Journal of Cell Biology·Tatiana IoukRichard W Wozniak
May 15, 2002·Molecular Biology of the Cell·Eunah Chung, Rey-Huei Chen
Apr 11, 2003·Molecular Biology of the Cell·Song-Tao LiuTim J Yen
Aug 10, 2000·Molecular Biology of the Cell·B B QuimbyA H Corbett
Mar 14, 1998·Molecular Biology of the Cell·F C Luca, M Winey
May 16, 1998·Molecular Biology of the Cell·A R Schutz, M Winey
Sep 11, 2002·Molecular Biology of the Cell·Cheryl D WarrenForrest A Spencer
Aug 20, 2003·Molecular Biology of the Cell·Tennessee J YoderTrisha N Davis
Jul 23, 2004·Molecular Biology of the Cell·Suzanne VigneronThierry Lorca
Dec 24, 2004·Molecular Biology of the Cell·Sandra E EncaladaBruce Bowerman
Jul 8, 2005·Molecular Biology of the Cell·Robert J ScottRichard W Wozniak
Sep 7, 2007·Molecular Biology of the Cell·Christopher KasbekHarold A Fisk
Oct 4, 2011·Molecular Biology of the Cell·Robert S HaganPeter K Sorger
May 4, 2012·Molecular Biology of the Cell·Friederike AlthoffChristian F Lehner

Related Concepts

Saccharomyces cerevisiae Proteins
Tumor Cells, Uncertain Whether Benign or Malignant
Spindle
Cell Cycle Proteins
Chimeric Proteins, Recombinant
Mitotic Spindle Apparatus
MAD1
MPS1 protein, S cerevisiae
BUB3 protein, S cerevisiae
BUB3 protein, human

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