PMID: 7522569Oct 12, 1994Paper

Activation of the cystic fibrosis transmembrane regulator by cyclic AMP is not correlated with inhibition of endocytosis

Biochimica Et Biophysica Acta
G F Santos, W W Reenstra

Abstract

Based on the observation (Bradbury et al. (1992) Am. J. Physiol. 262, C752-C759) that conditions known to activate the cystic fibrosis transmembrane regulator protein (CFTR) increase the rate of exocytosis and decrease the rate of endocytosis, it was proposed that activation of the CFTR may involved cAMP-dependent fusion of CFTR containing endosomes with the apical membrane. We have tested this hypothesis in two cell lines derived from epithelia that express defective chloride transport in cystic fibrosis (CF): the human colonic cell line, T84, and the tracheal cell line 9HTEo-. The dose-dependence of forskolin- and CPT-cAMP-induced inhibition of endocytosis were compared with the dose-dependence of chloride channel activation. Endocytosis was determined from the uptake of FITC-dextran, and assayed in purified endosomes. Chloride channel activity was measured from the rate of I-efflux. If the fusion hypothesis is correct: (1) concentrations of agonist that inhibit endocytosis should activate chloride channel activity, and (2) the relationship between endocytosis and channel activation should be similar for forskolin and CPT-cAMP. Results in both cell lines were inconsistent with these postulates, suggesting that either chloride...Continue Reading

References

Mar 1, 1992·The American Journal of Physiology·N A BradburyR J Bridges
Jan 1, 1992·The Journal of Clinical Investigation·G M DenningM J Welsh
Apr 24, 1992·Science·N A BradburyK L Kirk
Feb 1, 1990·The American Journal of Physiology·W I LencerD Brown
Aug 1, 1990·The American Journal of Physiology·C J VenglarikR A Frizzell
Jul 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P M Quinton
Feb 1, 1993·The American Journal of Physiology·L S PrinceR B Marchase

❮ Previous
Next ❯

Citations

Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·J BiwersiA S Verkman
Aug 2, 2001·The Journal of General Physiology·P ChenK D Gillis
May 4, 2000·American Journal of Physiology. Cell Physiology·J L DicksonT W Ecay
Jan 29, 1999·Physiological Reviews·N A Bradbury
Sep 26, 2000·American Journal of Physiology. Cell Physiology·G A GibsonO A Weisz
Dec 1, 1996·The American Journal of Physiology·A TakahashiR A Frizzell
Apr 1, 1995·The American Journal of Physiology·B IllekW W Reenstra

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.